GMP APC/Fire™ 750 anti-human CD8 Antibody, CD8, SK1

Pricing & Availability

Clone: SK1 (See other available formats)
Other Names: T8, Leu2
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK1_GMP_APCFire750_CD8_Antibody_030723 — Typical results from human peripheral blood lymphocytes stained either with SK1 APC/Fire™ 750 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See GMP APC/Fire™ 750 spectral data

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260228

100 tests

287€

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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details

Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

100 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics⁸, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References (PubMed link indicates BioLegend citation)

Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
Campanelli R, et al. 2002. Intl. Immunol. 14:39.
Evans RL, et al. 1981. Immunol. 78:544.
Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 925 View all products for this Gene ID
UniProt: View information about CD8 on UniProt.org

Documentation

Reviews

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Cell Surface Flow Cytometry Staining Protocol

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KIRAVIA Blue 520™ anti-human CD8	SK1	FC
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Spark Violet™ 500 anti-human CD8	SK1	FC
GMP APC/Fire™ 750 anti-human CD8	SK1	FC
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Alexa Fluor® 660 anti-human CD8a	SK1	FC
Spark Blue™ 515 anti-human CD8	SK1	FC
Spark Blue™ 574 anti-human CD8	SK1	FC
Spark Violet™ 538 anti-human CD8	SK1	FC
PE/Fire™ 810 anti-human CD8	SK1	FC
Spark YG™ 593 anti-human CD8	SK1	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 06/19/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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