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GMP Recombinant Human IL-34 (carrier-free)

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Other Names
Interleukin-34
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Human_IL-34_CF_GMP-RECOM_1_032123
GMP recombinant human IL-34 induces MCP-1 (CCL2) production in human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner. The ED50 for this effect is 4 - 20 ng/mL.
  • Human_IL-34_CF_GMP-RECOM_1_032123
    GMP recombinant human IL-34 induces MCP-1 (CCL2) production in human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner. The ED50 for this effect is 4 - 20 ng/mL.
  • Human_IL-34_CF_GMP-RECOM_2_032123
    Stability Testing for GMP Recombinant Human IL-34. Human IL-34 was aliquoted in 10mM Na2HPO4, 0.5M NaCl, pH 7.4 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to induce MCP-1 (CCL2) production in human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner. The ED50 for this effect is 4 - 20 ng/mL.
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577916 100 µg 2052€
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577914 25 µg 658€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Human IL-34 shares amino acid sequence similarity of 99.6%, 72% and 71% with chimpanzee, rat, and mouse IL-34, respectively. The IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 shows lack of consensus in the structural domain or motif, and does not share any sequence homology with M-CSF but it still binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have differing ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF and, as a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines. High levels of IL-34 has been detected in serum of patients with polycystic ovary syndrome and in patients with acute ischemic stroke.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human IL-34, amino acid (Asn21-Pro242) (Accession: # NM_152456.2), with carboxy terminus 10H tag, was expressed in CHO cells.
Molecular Mass
The 235 amino acid recombinant protein has a predicted molecular mass of 26.7 kD. The DTT-reduced protein migrates at approximately 40 kD, and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE. The predicted N-terminal amino acid is Asn.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.1 µm filtered protein solution is in 10mM Na2HPO4, 0.5M NaCl, pH 7.4.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg and 100 µg sizes are at 0.5 mg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human IL-34 induces MCP-1 (CCL2) production in human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner. The ED50 for this effect is 4 - 20 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Homodimer
Distribution

IL-34 mRNA is expressed in different tissues, including spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon.

Function
IL-34 increases monocyte's viability, induces macrophage proliferation, and synergizes with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony-forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. Human IL-34 promotes the osteoclast differentiation from peripheral blood mononucleated cells. In addition, systematic administration of IL-34 to mouse increases the number of CD11b+ cells and reduces bone mass. IL-1β and TNF-α induce the expression of interleukin-34 mRNA in osteoblast. IL-34 is induced in macrophages infected with equine infectious anemia virus (EIAV).
Interaction
Macrophages, monocytes, myeloid cells
Ligand/Receptor
CSF-1R
Bioactivity
Induces cytokine production in PBMCs.
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Clanchy F, et al. 2006. J Leukoc Biol. 79:757-66.
  2. Lin H, et al. 2008. Science. 320:807-11.
  3. Chihara T, et al. 2010. Cell Death Differ. 17:1917-27.
  4. Wei S, et al. 2010. J Leukoc Biol. 88:495-505.
  5. Eda H, et al. 2010. Cytokine. 52:215-20.
  6. Covaleda L, et al. 2010. Virology. 397:217-23.
  7. Chen Z, et al. 2011. PLoS One. 6:e18689.
  8. Cai H, et al. 2022. J Obstet Gynecol Res. 48:973.
  9. Huang X, et al. 2021. J Neuroimminol. 358:577652.
Gene ID
146433 View all products for this Gene ID
UniProt
View information about IL-34 on UniProt.org
Go To Top Version: 1    Revision Date: 04/24/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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