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LEAF™ Purified anti-human SCF Antibody

Pricing & Availability
Clone
Poly5200 (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor, KIT-ligand, Kitl, KL-1, mast cell grow factor (MGF), steel factor (SF), FPH2, SHEP7
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5200_LEAF_SCF_Block_022625
Recombinant human CD117-Fc chimera (black circles) binds to immobilized human SCF (Cat. No. 573904). LEAF™ purified anti-human SCF (Poly5200) (purple squares) inhibits the binding in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. This antibody blocks the binding of 0.025 µg/mL human CD117-Fc chimera to 1 µg/ml immobilized human SCF. The ND50 range: 0.04 - 0.3 µg/mL
  • Poly5200_LEAF_SCF_Block_022625
    Recombinant human CD117-Fc chimera (black circles) binds to immobilized human SCF (Cat. No. 573904). LEAF™ purified anti-human SCF (Poly5200) (purple squares) inhibits the binding in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. This antibody blocks the binding of 0.025 µg/mL human CD117-Fc chimera to 1 µg/ml immobilized human SCF. The ND50 range: 0.04 - 0.3 µg/mL
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520004 100 µg 280€
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Description

Stem Cell Factor (SCF) was initially synthesized as membrane-bound protein of 248 or 220 amino acids, depending on alternative splicing of exon 6. The 248 amino acid form contains a proteolytic cleavage site encoded by exon 6 which is cleaved from the cell to release an active soluble protein of 165 amino acid residues. Soluble SCF is glycosylated at both N-linked and O-linked sites. MMP-9 plays a physiological role in SCF release from the membrane, and this action plays a significant role in differentiating and mobilizing stem and progenitor cells from the bone marrow. SCF increases the proliferation of myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF/c-kit interaction in mast cells results in mast cell degranulation with release of mediators, such as histamine and inflammatory cytokines and chemokines. Activation of c-kit in dendritic cells regulates T helper cell differentiation and allergic asthma. In addition, SCF plays an important role in re-vascularization of ischemic limbs. Ischemia induces plasma elevation of SCF and thrombopoietin (TPO) and, in lower levels, GM-CSF and erythropoietin (EPO). SCF and TPO can induce the release of CXCL12 from platelets into plasma. This results in an extensive mobilization of CXCR4+VEGFR1+ cells (hemangiocytes), accelerating re-vascularization of the ischemic limbs. SCF binds to its receptor kit that belongs to the type III tyrosine kinase family, whose members include receptors for M-CSF and PDGF.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human SCF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the ability to block the binding of an experimentally-determined spike concentration of human CD117-Fc to immobilized human SCF (Cat. No. 573904)   The ND50 range for this effect is 0.04 - 0.3 µg/mL.  It is recommended that this reagent be titrated to confirm performance.

RRID
AB_3683469 (BioLegend Cat. No. 520004)

Antigen Details

Structure
Homodimer
Distribution

Embryonic Stem Cells, Hematopoietic stem and progenitors

Function
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Interaction
Mast cells, natural killer cells, dendritic cells, eosinophils, epithelial cells, endothelial cells, melanocytes, germ cells, cholangiocytes, platelets, myeloid leukaemia cells, and intestinal cells of Cajal.
Ligand/Receptor
c-kit (CD117)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Lu HS, et al. 1996. J Biol Chem. 271:11309-16.
  2. Heissig B, et al. 2002. Cell. 109:625-37.
  3. Jin DK, et al. 2006. Nat. Med. 12:557-67.
  4. Krishnamoorthy N, et al. 2008. Nat Med. 14:565-73.
  5. Ray P, et al. 2010. Ann N. Y. Acad. Sci. 1183:104-22.
Gene ID
4254 View all products for this Gene ID
UniProt
View information about SCF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02/26/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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