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- Regulatory Status
- RUO
- Other Names
- MMP-9, CXCL-9 (MIG), CCL-18 (PARC), CD163, MMP-2, Galectin-3 (LGALS3), CCL-20 (MIP-3α), VEGF, M-CSF, PDGF-BB, G-CSF, CCL-22 (MDC), YKL-40 (CHI3L1)
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- Product Citations
- publications
Macrophages are produced by differentiation of monocytes in response to an infection or tissue damage. They play important roles in the immune response and in the maintenance of tissue homeostasis, such as recognizing, engulfing, destroying, and clearing foreign or harmful substances including pathogens, dying or dead cells, cellular debris and tumor cells. Many tumors recruit monocytes from circulation and transform them into an immunosuppressive subset called Tumor-Associated Macrophages (TAMs). TAMs are highly plastic and can alter their phenotype between M1 pro-inflammatory and M2 anti-inflammatory according to their location and surrounding cytokine milieu in the tumor microenvironment. M1 macrophages are considered to be functioning in the recognition and destruction of cancer cells whereas M2 macrophages are thought to promote proliferation, angiogenesis, and metastasis of cancer cells. Therefore, the phenotypes of TAMs and the soluble factors produced by them influence various aspects of cancer progression and patient prognosis.
The LEGENDplex™ Human Tumor-Associated Macrophage Panel (13-plex) is a bead-based multiplex assay, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 key targets involved in monocyte differentiation and macrophage functions including MMP-9, CXCL-9, CCL18, CD163, MMP-2, Galectin-3, CCL20 (MIP-3α), VEGF, M-CSF, PDGF-BB, G-CSF, CCL22 (MDC), and YKL-40 (CHI3L1). This assay panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
Kit Contents
- Kit Contents
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- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Human Tumor-Associated Macrophage Panel Premixed Beads
- LEGENDplex™ Human Tumor-Associated Macrophage Panel Detection Antibodies
- LEGENDplex™ Human Tumor-Associated Macrophage Panel Standard
- LEGENDplex™ Matrix C, Lyophilized
- LEGENDplex™ SA-PE
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- Plate Sealers
- Filter Plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex - Panel
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
Antigen Details
- Cell Type
- Macrophages
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Innate Immunity
- Molecular Family
- Adhesion Molecules, Cytokines/Chemokines, Growth Factors, Tumor Suppressors
- Gene ID
- 81687 View all products for this Gene ID 17329 View all products for this Gene ID 6362 View all products for this Gene ID 9332 View all products for this Gene ID 4313 View all products for this Gene ID 3596 View all products for this Gene ID 6364 View all products for this Gene ID 7422 View all products for this Gene ID 1435 View all products for this Gene ID 5155 View all products for this Gene ID 1441 View all products for this Gene ID 6367 View all products for this Gene ID 1116 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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