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ELISA MAX™ Standard Set Mouse IL-10

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Regulatory Status
RUO
Other Names
IL-10 ELISA Kit
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IL-10_Mouse_ELISAMax_081712
  • IL-10_Mouse_ELISAMax_081712
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431411 5 plates 212€
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Description

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Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation.

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-10 Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
  2. Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
  3. Kubo T, et al. 2009. J. Exp Med. 206:1971. PubMed
  4. Asai A, et al. 2012. Immunobiology. 217:711. PubMed
  5. Baca Jones C, et al. 2014. PLoS One. 9:90855. PubMed
Product Citations
  1. Asai A, et al. 2012. Immunobiology. 217:711. PubMed
  2. Hasnat MA, et al. 2022. Immunol Cell Biol. 100:605. PubMed
  3. Rengachar P, et al. 2022. Biomolecules. 12: . PubMed
  4. Rengachar P, et al. 2023. Front Endocrinol (Lausanne). 13:1053879. PubMed
  5. Yan K, et al. 2022. Phytother Res. 36:2161. PubMed
  6. Yang JM, et al. 2022. Sci Adv. 8:eabo0183. PubMed
  7. Lundahl MLE, et al. 2022. Elife. 11:. PubMed
  8. Santos I, et al. 2020. Proc Natl Acad Sci U S A. 117:12281. PubMed
  9. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  10. Wang Y, et al. 2021. Andrologia. 53:e14239. PubMed
  11. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  12. Singh I, et al. 2018. Immunobiology. 223:549. PubMed
  13. Gomes MT, et al. 2022. J Biol Chem. 298:102034. PubMed
  14. Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
  15. Lin YZ, et al. 2022. Comput Struct Biotechnol J. 20:241. PubMed
  16. Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
  17. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  18. Hsieh PS, et al. 2021. Exp Ther Med. 188:21. PubMed
  19. Colaço HG, et al. 2021. Immunity. 54:53:00. PubMed
  20. Stravinskas Durigon T, et al. 2018. J Innate Immun. 10:279. PubMed
  21. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  22. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  23. Singh N, et al. 2016. J Immunol. 196: 1108 - 1122. PubMed
  24. Kumar RK, et al. 2021. Vascul Pharmacol. 138:106838. PubMed
  25. Egli N, et al. 2015. PLoS One. 10: 0142741. PubMed
  26. Lajqi T, et al. 2021. Int J Mol Sci. 22:. PubMed
  27. Fenoy I, et al. 2015. Exp Parasitol. 154: 47-50. PubMed
  28. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  29. Hu Y, et al. 2017. Biochim Biophys Acta. 10.1016/j.bbadis.2017.06.001. PubMed
  30. Fenoy IM, et al. 2015. Immunobiology. 22: 641-648. PubMed
  31. Jones C, et al. 2014. PLoS One. 9:90855. PubMed
  32. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  33. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  34. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: Phosphate-Buffered Saline (PBS), pH 7.4
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB High Sensitivity Substrate Solution - BioLegend Cat. No. 421501 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 5    Revision Date: 02/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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