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Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ D7
  • D7_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ D7
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108119 25 µg 81€
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108120 100 µg 184€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
  2. Aguilo F, et al. 2011. Blood. 117:5057. PubMed
  3. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  4. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  5. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  6. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  7. Vasquez EG, et al. 2022. Cell Stem Cell. 29:1213. PubMed
  8. Abe I, et al. 2022. Dev Cell. 57:2623. PubMed
  9. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  10. Qiu J, et al. 2022. STAR Protoc. 3:101828. PubMed
  11. Pisterzi P, et al. 2023. Hemasphere. 7:e823. PubMed
  12. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  13. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  14. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  15. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  16. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  17. Søndergaard E, et al. 2020. Cell Reports. 29(9):2756-2769.e6.. PubMed
  18. Ambrosi TH, et al. 2021. Nature. 597:256. PubMed
  19. Giordani L, et al. 2020. Molecular Cell. 74(3):609-621.e6.. PubMed
  20. Dusseault J, et al. 2016. Diabetes. 65: 2652 - 2666. PubMed
  21. Gao B, et al. 2018. Aging Cell. 3. PubMed
  22. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  23. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  24. Grassinger J, et al. 2009. Blood. 114:49:00. PubMed
  25. Zhou S, et al. 2020. Cell Res. 30:1063. PubMed
  26. Wosczyna MN, et al. 2021. Cell Stem Cell. 28(7):1323-1334.e8. PubMed
  27. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  28. Xiong X et al. 2018. Cell reports. 25(11):3006-3020 . PubMed
  29. Khateb M, et al. 2022. Cell Rep. 40:111219. PubMed
  30. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  31. Wosczyna MN et al. 2019. Cell Rep. 27(7):2029-2035 . PubMed
  32. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  33. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  34. Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed
  35. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  36. Nemeth M, et al. 2009. Stem Cells. 27:1109. PubMed
  37. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  38. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  39. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  40. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  41. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  42. Chu X, et al. 2021. Front Cell Dev Biol. 9:671841. PubMed
  43. Mueller A, et al. 2016. Nature. 540:276-279. PubMed
  44. Liang R, et al. 2020. Cell Stem Cell. 359:26. PubMed
  45. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  46. Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed
  47. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  48. Karamitros D, et al. 2015. Development. 142:70. PubMed
  49. Torreggiani E, et al. 2013. PLoS One. 8:75204. PubMed
  50. Barrett TJ, et al. 2021. Cell Rep. 36:109595. PubMed
  51. Wu X, et al. 2018. Cell. 172:423. PubMed
  52. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  53. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  54. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  55. Agarwal A, et al. 2008. Blood. 112:1960. PubMed
  56. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  57. Liu L et al. 2018. Cell stem cell. 23(4):544-556 . PubMed
  58. Caputo T, et al. 2020. Cell Mol Life Sci. . PubMed
  59. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  60. Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed
  61. Ambrosio F, et al. 2014. Free Radic Biol Med. 74:64. PubMed
  62. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  63. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  64. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  65. Sarshad AA, et al. 2018. Mol Cell. 71:1040. PubMed
  66. Yue F, et al. 2022. Cell Rep. 38:110267. PubMed
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RRID
AB_493273 (BioLegend Cat. No. 108119)
AB_493273 (BioLegend Cat. No. 108120)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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