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PE anti-Baculovirus envelope gp64 Antibody

Pricing & Availability
Clone
AcV1 (See other available formats)
Regulatory Status
RUO
Other Names
gp64, gp67, major budded virus envelope glycoprotein, AcOrf-128
Isotype
Mouse IgG2a, κ
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Product Citations
publications
AcV1_PE_baculovirus_gp64_Antibody_050317
SF9 cells infected with baculovirus (24 hours) were stained with gp64 (clone AcV1) PE (filled histogram) or mouse IgG2a, κ PE (open histogram).
  • AcV1_PE_baculovirus_gp64_Antibody_050317
    SF9 cells infected with baculovirus (24 hours) were stained with gp64 (clone AcV1) PE (filled histogram) or mouse IgG2a, κ PE (open histogram).
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250605 25 µg 142€
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250606 100 µg 240€
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Description

GP64 is the major envelope fusion protein of some, but not all, baculoviruses. GP64 is a homotrimeric membrane glycoprotein that is required for efficient budding of the virion and cell-to-cell transmission. This antibody can be used for detection of baculovirus infection and in quantifying viral infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Baculovirus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
AcNPV extracellular nonoccluded virus (NOV)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zhou J and Blissard GW. 2006. Virology 352:427.
  2. Kumar M, et al. 2003. Hum. Gene Ther. 14:67.
  3. Volkman LE, et al. 1988. Virology 166:285.
  4. Hohmann AW, et al. 1983. Virology 125:432.
RRID
AB_2687076 (BioLegend Cat. No. 250605)
AB_2687076 (BioLegend Cat. No. 250606)

Antigen Details

Structure
Homotrimeric membrane glycoprotein.
Function
Essential for efficient budding of virion and cell-to-cell transmission.
Biology Area
Immunology
Antigen References

1. Luz-Madrigal A, et al. 2013. J. Virol. 87:11894.
2. Dhungel B, et al. 2013. BMC Biotechnol. 13:27.
3. Oakland M, et al. 2013. Mol. Ther. Nucleic Acids 2:e69.

Gene ID
1403961 View all products for this Gene ID
UniProt
View information about Baculovirus envelope gp64 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 05/03/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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