PE anti-human CD1a Antibody anti-CD1a

Pricing & Availability

Clone: SK9 (See other available formats)
Regulatory Status: RUO
Other Names: T6, R4, Leu-6
Isotype: Mouse IgG2b, κ
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Product Citations: publications

SK9_PE_012110 — Human T lymphoblastic leukemia cell line Molt-4 stained with SK9 PE

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344904

100 tests

273€

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Description

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for the relevant formats) includes: immunoprecipitation and immunohistochemical staining of frozen sections.²
RRID: AB_2073130 (BioLegend Cat. No. 344904)

Antigen Details

Structure: Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Distribution: Cortical thymocytes, Langerhans cells, dendritic cells
Function: Antigen presentation, lymphocyte activation, thymic T cell development
Ligand/Receptor: CD1-restricted TCRs
Cell Type: Dendritic cells, Langerhans cells, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Wood GS, et al. 1983. J. Immunol. 131:212.
2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
3. Martin LH, et al. 1987. P. Natl. Acad. Sci. USA 84:9189.
Gene ID: 909 View all products for this Gene ID
UniProt: View information about CD1a on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Gamma-Delta T cells

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD1a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD1a	SK9	FC,IP,IHC-F
PE anti-human CD1a	SK9	FC
PE anti-human CD1a	SK9	FC
APC anti-human CD1a	SK9	FC
APC anti-human CD1a	SK9	FC
GMP APC anti-human CD1a	SK9	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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