PE anti-human CD257 BAFF, BLYS Antibody anti-CD257

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Clone: 1D6 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)
Isotype: Mouse IgG1, κ
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Product Citations: publications

1D6_PE_CD257_Antibody_FC_051115 — Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with anti-human CD257 (clone 1D6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

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366505

25 tests

132€

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366506

100 tests

227€

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Description

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD257 transfected murine cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2565708 (BioLegend Cat. No. 366505)
AB_2565708 (BioLegend Cat. No. 366506)

Antigen Details

Structure: Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.
Distribution: Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA/ionomycin treatment.
Function: Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Ligand/Receptor: BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR/BR3).
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family: CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References: 1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Moore PA, et al. 1999. Science 285:260.
3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680.
4. Yu G, et al. 2000. Nat. Immunol. 1:252.
5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.
Gene ID: 10673 View all products for this Gene ID
UniProt: View information about CD257 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

BAFF in B-Cell Signaling

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD257 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD257 (BAFF, BLYS)	1D6	FC,IHC-P
Biotin anti-human CD257 (BAFF, BLYS)	1D6	FC
PE anti-human CD257 (BAFF, BLYS)	1D6	FC
APC anti-human CD257 (BAFF, BLYS)	1D6	FC
APC/Cyanine7 anti-human CD257 (BAFF, BLYS)	1D6	FC
TotalSeq™-C0863 anti-human CD257 (BAFF, BLYS)	1D6	PG
TotalSeq™-B0863 anti-human CD257 (BAFF, BLYS)	1D6	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 05/12/2015

For Research Use Only. Not for diagnostic or therapeutic use.

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