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PE anti-human CD62E Antibody

Pricing & Availability
Clone
HAE-1f (See other available formats)
Regulatory Status
RUO
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HAE-1f_PE_040810
TNF-α-stimulated (6hr) HUVEC cells stained with CD62E (clone HAE-1f) PE
  • HAE-1f_PE_040810
    TNF-α-stimulated (6hr) HUVEC cells stained with CD62E (clone HAE-1f) PE
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336008 100 tests 215€
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162).The HAE-1f antibody has been shown to recognize human CD62E and to be useful for flow cytometry and in vitro blocking.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications: blocks CD62E binding to its ligand

Application References

(PubMed link indicates BioLegend citation)
  1. Tu L, et al. 1996. J. Immunol. 157:3995
  2. Zhang Y and S. Neelamegham. 2002. Biophys. J. 83:1934
Product Citations
  1. Li L, et al. 2022. FEBS J. 289:2877. PubMed
  2. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
  3. Mutoh T, et al. 2020. Nat Commun. 11:1253. PubMed
  4. Giannella A, et al. 2021. Cardiovasc Diabetol. 20:77. PubMed
  5. Jamaly S, et al. 2018. J Thromb Haemost. 16(8):1546. PubMed
RRID
AB_2186830 (BioLegend Cat. No. 336008)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells (IL-2, TNF-α), other cytokines can increase expression); can also be expressed on endothelial cells in the skin, bone marrow and placenta.

Function
Involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported in interact with CD62E
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
5. Walz G, et al. 1990. Science 250:1132

Gene ID
6401 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06/20/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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