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PE anti-human Ganglioside GD2 Antibody

Pricing & Availability
Clone
14G2a (See other available formats)
Regulatory Status
RUO
Other Names
Disialoganglioside GD2, Ganglioside G2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14G2a_PE_GD2_Antibody_FC_021913
Human melanoma cell line M21 was stained with ganglioside GD2 (clone 14G2a) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 14G2a_PE_GD2_Antibody_FC_021913
    Human melanoma cell line M21 was stained with ganglioside GD2 (clone 14G2a) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
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357303 25 tests 123€
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357304 100 tests 308€
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Description

Ganglioside GD2 is a sialic acid-containing glycosphingolipid involved in cell attachment to the extracellular matrix. Expression of GD2 in normal tissue is restricted to cells from the central nervous system, peripheral nerves, skin melanocytes, and mesenchymal stem cells. However GD2 is highly expressed by tumors of neuro-ectodermal origin such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma. GD2 has been proposed as a marker for some cancer stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Neuroblastoma cell line LAN-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3)1. Additional reported applications (for the relevant formats) include: inducing apoptosis and enhancing cytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line 2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact our custom solutions team). This clone has also been published as 14.G2a.

Application References

(PubMed link indicates BioLegend citation)
  1. Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt)
  2. Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt)
  3. Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC)
Product Citations
  1. Brignole C, et al. 2021. J Exp Clin Cancer Res. 40:180. PubMed
  2. Baniel CC, et al. 2020. J Immunother Cancer. 8:00. PubMed
  3. Baniel CC, et al. 2020. Front Immunol. 1.576388889. PubMed
  4. Richman SA, et al. 2018. Cancer Immunol Res. 6:36. PubMed
  5. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed
  6. Morris ZS, et al. 2018. Cancer Immunol Res. 6:825. PubMed
  7. Barton J, et al. 2019. F1000Res. 8:823. PubMed
  8. Strobel SB, et al. 2021. Life (Basel). 11:. PubMed
  9. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  10. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  11. Magee K, et al. 2021. PLoS One. 16:e0255798. PubMed
  12. Voeller J, et al. 2019. J Immunother Cancer. 0.530555556. PubMed
RRID
AB_2561884 (BioLegend Cat. No. 357303)
AB_2561884 (BioLegend Cat. No. 357304)

Antigen Details

Distribution

Neurons, mesenchymal stem cells, highly expressed in tumors of neuro-ectodermal origin, such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma

Function
Cell attachment to extracellular matrix
Cell Type
Mesenchymal cells, Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience
Molecular Family
Adhesion Molecules
Antigen References

1. Tarek N, et al. 2012. J. Clin. Invest. 122:3260.
2. Matthay KK, et al. 2012. Clin. Cancer Res. 18:2740.
3. Navid F, et al. 2010. Curr. Cancer Drug Targets. 10:200.

Gene ID
2583 View all products for this Gene ID
UniProt
View information about Ganglioside GD2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/21/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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