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PE anti-human/mouse Bcl-6 Antibody

Pricing & Availability
Clone
7D1 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7D1_PE_Bcl-6_Antibody_1_091924
11-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Pacific Blue™ and IgD PerCP/Cyanine5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) PE (left) or rat IgG2a, κ PE isotype control (right). Data shown was gated on B220+ positive cells.
  • 7D1_PE_Bcl-6_Antibody_1_091924
    11-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Pacific Blue™ and IgD PerCP/Cyanine5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) PE (left) or rat IgG2a, κ PE isotype control (right). Data shown was gated on B220+ positive cells.
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358503 25 tests 123€
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358504 100 tests 317€
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Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sequence 226-398 of murine BCL6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Vikstrom, et al. 2010. Science 330:1095. (WB)
  2. Kallies A, et al. 2011. Blood 117:1869. (WB)
  3. Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB)
  4. Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)
Product Citations
  1. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  2. Turner JS, et al. 2020. Nature. 586:127. PubMed
  3. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
  4. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
RRID
AB_2562152 (BioLegend Cat. No. 358503)
AB_2562152 (BioLegend Cat. No. 358504)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172.
2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671.
3. Kitano M, et al. 2011. Immunity 34:961.
4. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
5. Johnston RJ, et al. 2009. Science. 325:1006.
6. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/03/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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