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PE anti-human TNAP Antibody

Pricing & Availability
Clone
W8B2 (See other available formats)
Regulatory Status
RUO
Other Names
Tissue Non-specific Alkaline Phosphatase, mesenchymal stem cell antigen-1, MSC, W8B2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W8B2_PE_021808
Human retinoblastoma cell line Weri-RB cells stained W8B2 PE
  • W8B2_PE_021808
    Human retinoblastoma cell line Weri-RB cells stained W8B2 PE
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327305 25 tests 117€
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327306 100 tests 254€
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Description

The W8B2 antibody reacts with an antigen, TNAP (Tissue non-specific alkaline phosphatase), that is selectively expressed on bone marrow mesenchymal stem cells, but not on placenta-derived mesenchymal stem cells. It was previously reported that the antibody recognized MSCA-1, mesenchymal stem cell antigen-1. Mesenchymal stem cells or MSCs were originally named as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Retinoblastoma cell line (Weri-RB-1 cells)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Battula VL, et al. 2009. Haematologica. 94:173.
Product Citations
  1. Korody ML, et al. 2021. Stem Cells Dev. 30:177. PubMed
  2. Liang D, et al. 2022. Front Cell Dev Biol. 10:883314. PubMed
  3. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
RRID
AB_940677 (BioLegend Cat. No. 327305)
AB_940677 (BioLegend Cat. No. 327306)

Antigen Details

Distribution

Bone marrow mesenchymal stem cells

Cell Type
Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Battula VL, et al. 2007. Differentiation. 75:279.

Gene ID
249 View all products for this Gene ID
UniProt
View information about TNAP on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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