PE anti-mouse CD138 (Syndecan-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse mammary gland epithelial cell line NMuMG

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue³ and formalin-fixed paraffin embedded tissue⁴ and immunofluorescent staining^2,3.

Application References

(PubMed link indicates BioLegend citation)

1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)

Product Citations

1. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
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12. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
13. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
14. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
15. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
16. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
17. Pan H, et al. 2020. Mol Psychiatry. . PubMed
18. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
19. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
20. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
21. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
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23. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
24. Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed
25. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
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27. Roy K, et al. 2019. Immunity. 50:616. PubMed
28. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
29. Li H, et al. 2020. Nature. 584:274. PubMed
30. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
31. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
32. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
33. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
34. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
35. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
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38. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
39. Schager AE, et al. 2018. MBio. 9:02379. PubMed
40. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
41. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
42. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
43. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
44. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
45. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
46. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
47. Che N, et al. 2021. Front Immunol. 12:626310. PubMed
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49. Steinmetz TD, et al. 2021. Autophagy. 17:2238. PubMed
50. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed

RRID

AB_10916119 (BioLegend Cat. No. 142503)
AB_10916119 (BioLegend Cat. No. 142504)

Antigen Details

Structure

Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD

Function

Cell adhesion, cell motility, cell migration, cell proliferation

Cell Type

B cells

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID

20969 View all products for this Gene ID

UniProt

View information about CD138 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis