Loading...

PE anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_PE_062310
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 6D5_PE_062310
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 6D5_PE_CD19_Antibody_042922
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (solid line) or rat IgG2a, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
115507 50 µg 36€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115508 200 µg 108€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  3. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  4. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Wang Q, et al. 2020. Radiat Environ Biophys. 59:89. PubMed
  6. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  7. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  8. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  9. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  10. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  11. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  12. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  13. Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed
  14. Li X, et al. 2022. Cell. 185:1709. PubMed
  15. Dhalech AH, et al. 2022. J Virol. 96:e0123222. PubMed
  16. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  17. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  18. Bhatia V, et al. 2023. Nat Commun. 14:2041. PubMed
  19. He Y, et al. 2023. Biomolecules. 13:. PubMed
  20. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  21. Murphy C, et al. 2012. Infect Immun . 80:2712. PubMed
  22. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  23. Gawish R, et al. 2022. Elife. 11:. PubMed
  24. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  25. Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed
  26. Silver AC, et al. 2018. Heliyon. 4:e00579. PubMed
  27. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  28. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  29. Culina S, et al. 2014. PLoS One. 9:88365. PubMed
  30. Hiraishi Y, et al. 2018. Sci Rep. 8:18052. PubMed
  31. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  32. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  33. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  34. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  35. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  36. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  37. Yamaguchi T, et al. 2010. Genes Dev. 24:455. PubMed
  38. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  39. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  40. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  41. Hu EY, et al. 2021. Blood Adv. 5:3152. PubMed
  42. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  43. Benet Z, et al. 2021. Cell Reports. 34(6):108733. PubMed
  44. Tong Q, et al. 2022. FEBS Open Bio. 12:1542. PubMed
  45. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  46. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  47. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  48. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
  49. Yang H, et al. 2022. Nutrients. 14:. PubMed
  50. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  51. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
  52. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  53. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  54. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  55. Cho K, et al. 2021. iScience. 24:103117. PubMed
  56. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  57. Ruiz–Manzano RA, et al. 2020. Frontiers in Oncology. 0.927777778. PubMed
  58. Ghandhi SA, et al. 2019. Sci Rep. 9:19434. PubMed
  59. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  60. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  61. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  62. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  63. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  64. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  65. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  66. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  67. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  68. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  69. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  70. Raoof R, et al. 2021. J Neurosci. 41:8249. PubMed
  71. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  72. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  73. Muenchow L, et al. 2014. Immunol Lett. 160:113. PubMed
  74. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  75. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  76. Shu Z, et al. 2020. Mar Drugs. 18:00. PubMed
  77. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  78. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  79. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  80. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  81. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  82. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  83. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  84. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  85. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  86. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  87. Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed
  88. Aulova KS, et al. 2022. Molecules. 27:. PubMed
  89. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  90. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  91. Aalipour A, et al. 2020. Mol Ther Oncolytics. 17:232. PubMed
  92. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  93. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
  94. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  95. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  96. Schubert M, et al. 2021. Cancers (Basel). 13: . PubMed
  97. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  98. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  99. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  100. Wali S, et al. 2020. Am J Respir Cell Mol Biol. 758:63. PubMed
  101. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  102. Syres K, et al. 2009. Blood. 114:2452. PubMed
  103. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  104. Gawish R, et al. 2022. Elife. 11:. PubMed
  105. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  106. Yang H, et al. 2022. Viruses. 14:. PubMed
RRID
AB_313642 (BioLegend Cat. No. 115507)
AB_313642 (BioLegend Cat. No. 115508)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account