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PE anti-mouse CD284 (TLR4) Antibody

Pricing & Availability
Clone
SA15-21 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Isotype
Rat IgG2a, κ
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Product Citations
publications
SA15-21_PE_TLR4_Antibody_FC_021913
Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • SA15-21_PE_TLR4_Antibody_FC_021913
    Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
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145403 25 µg 98€
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145404 100 µg 210€
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Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1 Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
  2. Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
  3. Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
  4. Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)
Product Citations
  1. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  2. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  3. Ding L, et al. 2023. Immunology. 168:49. PubMed
  4. Rohwedder I, et al. 2023. JCI Insight. 8: . PubMed
  5. Schappe MS, et al. 2018. Immunity. 48:59. PubMed
  6. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  7. Li Y, et al. 2019. J Biol Chem. 294:2744. PubMed
  8. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  9. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  10. Hu J, et al. 2020. Theranostics. 7.154166667. PubMed
  11. Xu X, et al. 2018. Cell. 175:1336. PubMed
  12. Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed
  13. Miura I, et al. 2021. Physiol Rep. 9:e14859. PubMed
  14. Cilenti F, et al. 2021. Immunity. . PubMed
  15. Yang L, et al. 2015. PLoS One. 10:126576. PubMed
  16. Wei L, et al. 2015. J Biol Chem. 290: 16633 - 16652. PubMed
  17. Zanoni I et al. 2017. Immunity. 47(4):697-709 . PubMed
  18. Noya V, et al. 2017. Sci Rep. 7:40615. PubMed
  19. Kim K, et al. 2015. Infect Immun . 83:1150. PubMed
  20. Arya SB, et al. 2018. J Biol Chem. 293:9892. PubMed
RRID
AB_2561873 (BioLegend Cat. No. 145403)
AB_2561873 (BioLegend Cat. No. 145404)

Antigen Details

Structure
Type I transmembrane protein complex
Distribution
Macrophages, B cells and T cells
Interaction
Activates the innate immune system against Gram-negative pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
Macrophages, B cells, T cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.

Gene ID
21898 View all products for this Gene ID
UniProt
View information about CD284 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 02/07/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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