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PE anti-mouse/human CD282 (TLR2) Recombinant Antibody

Pricing & Availability
Clone
QA16A01 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor 2, TLR-2, TIL4, CD282, TLR2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA16A01_PE_CD282_Antibody_021218
Human peripheral blood monocytes were stained with PE anti-mouse/human CD282 (clone QA16A01, filled histogram) or PE mouse IgG1, κ isotype control (open histogram).
  • QA16A01_PE_CD282_Antibody_021218
    Human peripheral blood monocytes were stained with PE anti-mouse/human CD282 (clone QA16A01, filled histogram) or PE mouse IgG1, κ isotype control (open histogram).
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153003 25 µg 113€
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153004 100 µg 244€
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Description

CD282 (Toll-like receptor 2, TLR2) is an 85kDa, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular domain and a toll/IL-1R (TIR) in the cytoplasmic region. TLR2 is expressed by monocytes/macrophages, dendritic cells, granulocytes, endothelial cell and epithelial cells. TLR2 associates with TLR1 and TLR6 in the cell surface and is involved in the recognition of pathogen-associated molecular patterns (PAMPs) such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, and LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant extracellular domain of murine TLR2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Blocking of IL-6 and TNF-a production induced by P3CSK4 on RAW264.7 cells was observed during development.

Product Citations
  1. Wang J, et al. 2023. PLoS Pathog. e1011754:19. PubMed
  2. Soh TK, et al. 2020. Cell Rep. 33:108235. PubMed
RRID
AB_2728204 (BioLegend Cat. No. 153003)
AB_2728204 (BioLegend Cat. No. 153004)

Antigen Details

Structure
Type I transmembrane protein, 14 leucine-rich repeats (LRR) in the extracellular domain, the intracellular domain contains a toll/IL-1R (TIR) domain.
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells.

Function
Activates innate host defense, induces production of pro-inflammatory cytokines.
Interaction
TLR1, TLR6 , MYD88 and TRAF6.
Ligand/Receptor
Peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Biswas S, et al. 2016. Blood. 127:2618.
2. Wang Y, et al. 2016. Proc. Natl. Acad. Sci. USA 113:884.
3. Ramstead AG, et al. 2016. Infect. Immun. 84:940.
4. Scheeren FA, et al. 2014. Nat. Cell. Biol. 16:1238.
5. Barbalat R, et al. 2009. Nat. Immunol. 10:1200. 

Gene ID
24088 View all products for this Gene ID 7097 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 02/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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