- Clone
- A1/Ly49A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly49A
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
138703 | 25 µg | 69€ |
Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The A1/Ly49A antibody preferentially reacts with CRD of Ly-49AB6 alloantigen and has also shown cross-reaction with Ly-49ANOD, Ly-49PNOD and Ly-49P129/J.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse C57BL/6N T lymphoma EL-4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Nagasawa R, et al. 1987. J. Immunol. 138:815. (IP)
- Guy-Grand D, et al. 1996. Eur. J. Immunol. 26:2248. (FC)
- Zimmer J, et al. 2001. J. Exp. Med. 194:1531. (FC)
- Product Citations
-
- RRID
-
AB_2134787 (BioLegend Cat. No. 138703)
Antigen Details
- Structure
- C-type lectin, Ly-49 family, ITIM, 44 kD
- Distribution
-
NK cells, NKT cells
- Function
- Inhibitory NK cell receptor
- Ligand/Receptor
- H-2Dd, H-2Dk, H-2Dp
- Cell Type
- NK cells, NKT cells
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Silver ET, et al. 2000. J. Immunol. 165:1771. - Gene ID
- 16627 View all products for this Gene ID
- UniProt
- View information about Ly-49AB6 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Ly-49AB6 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse Ly-49AB6 | A1/Ly49A | FC |
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PE anti-mouse Ly-49AB6
C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1...
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