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PE anti-mouse MAdCAM-1 Antibody

Pricing & Availability
Clone
MECA-367 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosal addressin cell adhesion molecule-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MECA-367_PE_MAdCAM-1_Antibody_071416
TNFα-stimulated bEND.3 cells were stained with anti-mouse MAdCAM-1 (clone MECA-367) PE (filled histogram) or mouse IgG2a, κ isotype control PE (open histogram).
  • MECA-367_PE_MAdCAM-1_Antibody_071416
    TNFα-stimulated bEND.3 cells were stained with anti-mouse MAdCAM-1 (clone MECA-367) PE (filled histogram) or mouse IgG2a, κ isotype control PE (open histogram).
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120709 25 µg 74€
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120710 100 µg 172€
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Description

MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Endothelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Streeter PR, et al. 1988. Nature 331:41.
  2. Briskin MJ, et al. 1993. Nature 363:461.
  3. Berlin C, et al. 1993. Cell 74:185.
  4. Bargatze RF, et al. 1995. Immunity 3:99.
  5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
  6. Savinov AY, et al. 2003. J. Exp. Med. 197:643.
  7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
  8. Hindley JP, et al. 2012. Cancer Res. 72:5473. PubMed.
Product Citations
  1. Pagano S, et al. 2023. J Transl Med. 694:21. PubMed
RRID
AB_2629559 (BioLegend Cat. No. 120709)
AB_2629559 (BioLegend Cat. No. 120710)

Antigen Details

Structure
Type I glycoprotein, Ig superfamily
Distribution

HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas

Function
Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand/Receptor
Integrin a4ß7, CD62L
Cell Type
Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.

Gene ID
17123 View all products for this Gene ID
UniProt
View information about MAdCAM-1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/14/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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