- Clone
- SH1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Siglec10, Sialic acid-binding Ig-like lectin G
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
163303 | 25 µg | 142€ | ||||
163304 | 100 µg | 308€ |
Siglec-G is a member of the CD33-related Siglec (sialic acid–binding, immunoglobulin-like lectin) family. Siglec-G is the orthologue of human Siglec-10. It is a 110-120 kDa, 688 amino acid type I transmembrane protein and contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs. It is an inhibitory receptor that is expressed in a B cell–restricted way, with particularly high expression in Pre-B and B1a cells. Upon stimulation with an antigen, Siglec-G is recruited to IgM, which is further phosphorylated inhibiting IgM-induced Ca2+ signaling and NFATc1 expression.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_3662201 (BioLegend Cat. No. 163303)
AB_3662201 (BioLegend Cat. No. 163304)
Antigen Details
- Structure
- Type I transmembrane protein that contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs.
- Distribution
-
B cells
- Function
- Inhibitis IgM-induced Ca2+ signaling and NFATc1 expression
- Ligand/Receptor
- Lectin
- Gene ID
- 243958 View all products for this Gene ID
- UniProt
- View information about Siglec-G on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Siglec-G Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Siglec-G Antibody | SH1 | FC |
PE anti-mouse Siglec-G | SH1 | FC |
Alexa Fluor® 647 anti-mouse Siglec-G | SH1 | FC |
Spark UV™ 387 anti-mouse Siglec-G | SH1 | FC |
Brilliant Violet 421™ anti-mouse Siglec-G | SH1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse Siglec-G Antibody
C57 splenocytes were stained with purified Siglec-G (clone S... -
PE anti-mouse Siglec-G
C57BL/6 splenocytes were stained with anti-mouse CD19 (clone... -
Alexa Fluor® 647 anti-mouse Siglec-G
C57BL/6 splenocytes were stained with anti-mouse CD19 (clone... -
Spark UV™ 387 anti-mouse Siglec-G
C57BL/6 splenocytes were stained with anti-mouse CD19 (clone... -
Brilliant Violet 421™ anti-mouse Siglec-G
C57BL/6 splenocytes were stained with anti-mouse CD19 (clone...
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