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PE anti-mouse TCR Vβ2 Antibody

Pricing & Availability
Clone
B20.6 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vβ 2 [vb2]
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
B20.6_PE_1_080812
C57BL/6 mouse splenocytes were stained with CD3 APC and TCR Vβ2 (clone B20.6) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • B20.6_PE_1_080812
    C57BL/6 mouse splenocytes were stained with CD3 APC and TCR Vβ2 (clone B20.6) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • B20.6_PE_2_080812
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127907 25 µg 79€
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127908 100 µg 157€
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Description

The B20.6 antibody recognizes V beta 2 TCR of all strains tested. V beta 2 bearing T lymphocytes are clonally eliminated in MHC class II I-E expressing mice neonatally infected with MMTV virus through milk feeding.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone CW3/1.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Necker A, et al. 1991. Eur J. Immunol. 21:3035
  2. Hodes RJ, et al. 1993. J Immunol 150:1422
Product Citations
  1. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
  2. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  3. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
RRID
AB_1227785 (BioLegend Cat. No. 127907)
AB_1227785 (BioLegend Cat. No. 127908)

Antigen Details

Structure
TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution

On a subset of T cells

Function
Recognize MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with V alpha and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Necker A, et al. 1991. Eur. J. Immunol. 21:3035

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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