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PE anti-rat CD172a (SIRPα) Antibody

Pricing & Availability
Clone
OX-41 (See other available formats)
Regulatory Status
RUO
Other Names
SIRPa, PTPNS1, BIT, MFR, p84, CD172 antigen-like family member A
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-41_PE_011409.jpg
LOU rat bone marrow cells stained with OX-41 PE (gated on granulocyte population)
  • OX-41_PE_011409.jpg
    LOU rat bone marrow cells stained with OX-41 PE (gated on granulocyte population)
See PE spectral data
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204706 100 µg 124€
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Description

Rat CD172a, also known as a signal-regulatory protein α (SIRPα), is a 75-110 kD transmembrane glycoprotein involved in the receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily primarily expressed on monocytes/macrophages, granulocytes, dendritic cells and neurons. CD172a serves as a substrate of activated receptor ttyrosine kinases (RTKs). The interaction of the CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion and phagocytosis. CD47 (IAP) is an extracellular ligand for CD172a.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat peritoneal macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting3, immunoprecipitation1, and immunohistochemical staining1, 2.

Application References

(PubMed link indicates BioLegend citation)
  1. Robinson AP, et al. 1986. Immunology 57:239. (IP, IHC)
  2. Thai QA, et al. 1999. Stroke 30:140. (IHC)
  3. Adams S, et al. 1998. J. Immunol. 161:1853. (WB)
Product Citations
  1. Liu C, et al. 2022. J Inflamm Res. 15:2803. PubMed
  2. Morandi B, et al. 2013. J Immunol. 191:4858. PubMed
RRID
AB_2188080 (BioLegend Cat. No. 204706)

Antigen Details

Distribution

Monocytes/macrophages, granulocytes, dendritic cells and neurons

Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Robinson AP, et al. 1986. Immunology 57:239.
2. Thai QA, et al. 1999. Stroke 30:140.
3. Adams S, et al. 1998. J. Immunol. 161:1853.

Gene ID
19261 View all products for this Gene ID
UniProt
View information about CD172alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10/25/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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