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PE anti-rat CD62L Antibody

Pricing & Availability
Clone
OX-85 (See other available formats)
Regulatory Status
RUO
Other Names
LECAM-1, Ly-22, LAM-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
OX-85_PE_011909.jpg
LOU rat splenocytes stained with OX-85 PE and anti-rat CD3 (1F4) Alexa Fluor® 647
  • OX-85_PE_011909.jpg
    LOU rat splenocytes stained with OX-85 PE and anti-rat CD3 (1F4) Alexa Fluor® 647
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202912 100 µg 200€
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Description

CD62L is a 74-95 kD glycoprotein,also known as L-selectin, LECAM-1, Ly-22 and MEL-14.It is a member of the selectin family and is expressed on the majority of B and naïve T cells,a subset of memory T cells,granulocytes ,subsets of thymocytes and NK cells.CD62L is important in lymphocyte homing to high endothelial venules in peripheral lymph nodes and leukocyte “rolling” on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites.CD62L is rapidly shed from lymphocytes and neutrophils upon activation and the expression levels of CD62L (in conjunction with other markers) hae been used to distinguish naïve, effector and memory T cells.CD62L has been reported to interact with )-glycans presented on CD34, GlyCAM-1, and MadCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat purified CD62L-CD4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vosine C. 2002 J. Immunol. 169:2284.
Product Citations
  1. Schläger C, et al. 2016. Nature. 530:349-353. PubMed
  2. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
RRID
AB_2270062 (BioLegend Cat. No. 202912)

Antigen Details

Structure
74-95 Kd glycoprotein of selectin family.
Distribution

Expressed on majority of B cell and naïve T cell and other leukocytes

Function
Homing and emigration of leukocytes
Cell Type
B cells, Leukocytes, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Regulation
Decreased expression upon cell activation
Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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