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PE/Cyanine7 anti-GATA3 Antibody

Pricing & Availability
Clone
16E10A23 (See other available formats)
Regulatory Status
RUO
Other Names
GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_16E10A23_PECyanine7_GATA3_Antibody_1_080323
Th2 polarized human peripheral blood lymphocytes were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) then stained with anti-GATA3 (clone 16E10A23) PE/Cyanine7 (left) or mouse IgG2b, κ PE/Cyanine7 isotype control (right). Cells were co-stained with anti-human CD3 APC.
  • A_16E10A23_PECyanine7_GATA3_Antibody_1_080323
    Th2 polarized human peripheral blood lymphocytes were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) then stained with anti-GATA3 (clone 16E10A23) PE/Cyanine7 (left) or mouse IgG2b, κ PE/Cyanine7 isotype control (right). Cells were co-stained with anti-human CD3 APC.
  • B_16E10A23_PECyanine7_GATA3_Antibody_2_080323
    Murine Th2 cell line D10.G4.1 was treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) then stained with anti-GATA3 (clone 16E10A23) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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653819 25 tests 128€
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653820 100 tests 284€
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Description

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines, responsible for preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defect in GATA3 results in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human GATA3 recombinant protein (1-258 aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3083421 (BioLegend Cat. No. 653819)
AB_3083421 (BioLegend Cat. No. 653820)

Antigen Details

Structure
48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.
Distribution

Nucleus

Function
GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation.
Interaction
Interacts with Smad3, FOG1, FOG2, and PU.1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Yagi R, et al. 2011. Int. Immunol. 23:415.
2. Chou J, et al. 2010. J. Cell Physiol. 222:42.
3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125.
4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236.
5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164.
6. Van Esch H, et al. 2000. Nature 406:419.

Gene ID
2625 View all products for this Gene ID 14462 View all products for this Gene ID
UniProt
View information about GATA3 on UniProt.org
Go To Top Version: 1    Revision Date: 08/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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