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PE/Cyanine7 anti-mouse CCL5 (RANTES) Antibody

Pricing & Availability
Clone
2E9/CCL5 (See other available formats)
Regulatory Status
RUO
Other Names
CCL5, SISd, Scya5, RANTES, TCP228
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2E9-CCL5_PECyanine7_CCL5_Antibody_052820
Overnight cultured C57BL/6 splenocytes were stained with CD8a, CD3, and CD44. Then, they were fixed, permeabilized, and intracellularly stained with CCL5 (clone 2E9/CCL5) PE/Cyanine7 (left) or mouse IgG2b, κ PE/Cyanine7 (right) isotype control. Data shown is gated on CD8a+ CD3+ population.
  • 2E9-CCL5_PECyanine7_CCL5_Antibody_052820
    Overnight cultured C57BL/6 splenocytes were stained with CD8a, CD3, and CD44. Then, they were fixed, permeabilized, and intracellularly stained with CCL5 (clone 2E9/CCL5) PE/Cyanine7 (left) or mouse IgG2b, κ PE/Cyanine7 (right) isotype control. Data shown is gated on CD8a+ CD3+ population.
See PE/Cyanine7 spectral data
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149105 25 µg 141€
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149106 100 µg 306€
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Description

Chemokine (C-C motif) ligand 5 (CCL5), also known as regulated on activation, normal T cell expressed and secreted (RANTES), is a 10 kD protein primarily produced by CD8+ T cells. CCL5 can also be secreted by platelets, dendritic cells (DCs), and fibroblasts. CCL5 functions as a chemoattractant for T cells, monocytes, and eosinophils through binding of its receptor, CCR5. CCL5 can inhibit binding of HIV-1 to CCR5 and inhibit HIV-1 entry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant CCL5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Jubin V, et al. 2012. Immunol. Res. 52:284.
Product Citations
  1. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
RRID
AB_2832528 (BioLegend Cat. No. 149105)
AB_2832528 (BioLegend Cat. No. 149106)

Antigen Details

Structure
Chemokine (C-C motif) ligand 5, 10 kD
Distribution

CD8+ T cells, platelets, DCs, and fibroblasts.

Function
Chemoattractant for T cells, monocytes, and eosinophils.
Ligand/Receptor
CCR5, CCR3, and CCR1.
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schall TJ. 1991. Cytokine 3:165.
2. Schall TJ, et al. 1992. Eur. J. Immunol. 6:1477.
3. Son MH, et al. 2014. Biochem. Biophys. Res. Commun. 450:30.

Gene ID
20304 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org
Go To Top Version: 1    Revision Date: 01/24/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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