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PE/Cyanine7 anti-mouse CD105 Antibody

Pricing & Availability
Clone
MJ7/18 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Rat IgG2a, κ
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Product Citations
publications
MJ7slash18_PECy7_040810
BEND.3 mouse endothelial cells stained with MJ7/18 PE/Cyanine7
  • MJ7slash18_PECy7_040810
    BEND.3 mouse endothelial cells stained with MJ7/18 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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120409 25 µg 118€
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120410 100 µg 278€
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Description

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Inflamed mouse skin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Ge AZ and Butcher EC. 1994. Gene 138:201.
  2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
  3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
  4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  3. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  4. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  5. Feng H, et al. 2020. J Clin Invest. 130:6354. PubMed
  6. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  7. Derby B, et al. 2014. Aesthet Surg J. 34:142. PubMed
  8. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  9. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  10. Szade K, et al. 2013. PLoS One. 8:63329. PubMed
  11. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  12. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  13. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  14. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  15. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  16. Jumabay M, et al. 2022. Sci Rep. 12:5614. PubMed
  17. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  18. Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed
  19. Farsaci B, et al. 2014. Cancer Immunol Res. 2:1090. PubMed
  20. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  21. Jing Y, et al. 2020. Cancer Gene Ther. 27:910. PubMed
  22. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  23. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  24. Labedz-Maslowska A, et al. 2015. PLoS One. 10: 0133746. PubMed
  25. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  26. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  27. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  28. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  29. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  30. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_1027700 (BioLegend Cat. No. 120409)
AB_1027700 (BioLegend Cat. No. 120410)

Antigen Details

Structure
Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution

Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast

Function
Suppresses TGF-β signaling, angiogenesis
Ligand/Receptor
TGF-β1, TGF-β3
Cell Type
Endothelial cells, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.

Gene ID
13805 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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