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PE/Cyanine7 anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_PECy7_081908.jpg
SJL splenocytes stained with A20 PE/Cyanine7
  • A20_PECy7_081908.jpg
    SJL splenocytes stained with A20 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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110729 25 µg 53€
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110730 100 µg 168€
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Burghardt S, et al. 2013. J Immunol. 191:5574. PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  4. du Halgouet A, et al. 2023. Immunity. 56:78. PubMed
  5. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  6. Pasqual G, et al. 2023. Methods Mol Biol. 2618:71. PubMed
  7. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  8. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  9. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  10. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  11. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  12. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  13. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  14. Puiffe ML, et al. 2020. Front Immunol. 11:600012. PubMed
  15. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  16. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
  17. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  18. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  19. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  20. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  21. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  22. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  23. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  24. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  25. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  26. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  27. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  28. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  29. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  30. Geng S, et al. 2021. JCI Insight. 6:. PubMed
  31. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  32. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  33. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  34. Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed
  35. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  36. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  37. Subedi A, et al. 2021. Cell Stem Cell. :. PubMed
  38. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  39. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  40. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  41. Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed
  42. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  43. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  44. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  45. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  46. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  47. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  48. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  49. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  50. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  51. Koide S, et al. 2022. iScience. 25:103603. PubMed
  52. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  53. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  54. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  55. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  56. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  57. Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed
  58. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  59. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  60. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  61. Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed
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RRID
AB_1134170 (BioLegend Cat. No. 110729)
AB_1134170 (BioLegend Cat. No. 110730)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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