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PE/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_PECy7_092409
C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
  • 1A8_PECy7_092409
    C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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127617 25 µg 80€
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127618 100 µg 243€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  2. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  3. Buckland RL, et al. 2020. Curr Protoc Mouse Biol. 10:e78. PubMed
  4. Zhang Y, et al. 2022. J Cereb Blood Flow Metab. 42:952. PubMed
  5. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  6. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  7. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  8. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  9. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  10. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  11. Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed
  12. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  13. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  14. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  15. Schick J, et al. 2023. Elife. 12: . PubMed
  16. Hu R, et al. 2023. Cell Death Discov. 9:50. PubMed
  17. Furuya Y, et al. 2014. J Virol. 88:9166. PubMed
  18. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  19. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  20. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  21. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  22. Solier S, et al. 2023. Nature. 617:386. PubMed
  23. Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed
  24. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  25. Zhao C, et al. 2023. NPJ Regen Med. 8:29. PubMed
  26. Huang T, et al. 2023. Cell Death Dis. 14:354. PubMed
  27. Gawish R, et al. 2022. Elife. 11:. PubMed
  28. Israelow B, et al. 2020. bioRxiv. . PubMed
  29. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
  30. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  31. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  32. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  33. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  34. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  35. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  36. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  37. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  38. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  39. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  40. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  41. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  42. Douglas T, et al. 2015. J Immunol. 195: 2365-2373. PubMed
  43. Mercer HL, et al. 2020. PLoS Pathog. 16:e1008572. PubMed
  44. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  45. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  46. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  47. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  48. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  49. Sanfilippo A, et al. 2015. Infect Immun . 83: 2976-2983. PubMed
  50. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  51. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  52. Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed
  53. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  54. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  55. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  56. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  57. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  58. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
  59. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  60. Lu S, et al. 2020. JCI Insight. 5:00. PubMed
  61. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
  62. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  63. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  64. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  65. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  66. Yu X, et al. 2022. J Extracell Vesicles. 11:e12185. PubMed
  67. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  68. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  69. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  70. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  71. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  72. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  73. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  74. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  75. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  76. Singh S, et al. 2021. Cell Host Microbe. 29(3):347-361.e12. PubMed
  77. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  78. Chan C, et al. 2012. Hypertension. 60:1207. PubMed
  79. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  80. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  81. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  82. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  83. Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed
  84. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  85. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
  86. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  87. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  88. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  89. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  90. Nguyen GT, et al. 2021. iScience. 24(8):102871. PubMed
  91. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  92. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  93. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  94. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  95. Yang Y, et al. 2022. Elife. 11:. PubMed
  96. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  97. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  98. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  99. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  100. Kaufmann E et al. 2018. Cell. 172(1-2):176-190 . PubMed
  101. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  102. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  103. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  104. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  105. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  106. Lei J, et al. 2021. Theranostics. 4251:11. PubMed
  107. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  108. Bryant J, et al. 2014. J Immunol. 192:6092. PubMed
  109. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
  110. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  111. Bravo-Caparrós I, et al. 2020. FASEB J. 34:5951. PubMed
  112. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  113. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  114. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  115. Chen F, et al. 2016. J Immunol. 196: 4390 - 4399. PubMed
  116. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  117. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  118. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  119. Ngamsri KC, et al. 2020. Front Immunol. 11:407. PubMed
  120. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  121. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  122. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  123. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
  124. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  125. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  126. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  127. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  128. Wang X, et al. 2021. Cell. 184:5357. PubMed
  129. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  130. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  131. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  132. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  133. Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
  134. Tang S, et al. 2022. Virol J. 19:32. PubMed
  135. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  136. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  137. Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed
  138. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  139. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  140. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  141. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  142. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  143. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  144. Braza MS et al. 2018. Immunity. 49(5):819-828 . PubMed
  145. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  146. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  147. Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed
  148. Lee J, et al. 2015. Nat Commun. 6: 8499. PubMed
  149. Goc J, et al. 2021. Cell. 184:5015. PubMed
  150. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  151. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  152. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  153. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  154. Wu L, et al. 2021. Cell Death Dis. 12:1064. PubMed
  155. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  156. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
RRID
AB_1877261 (BioLegend Cat. No. 127617)
AB_1877261 (BioLegend Cat. No. 127618)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 3    Revision Date: 01/06/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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