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PE/Dazzle™ 594 anti-human CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
24F.10C12 (See other available formats)
Regulatory Status
RUO
Other Names
B7-DC, PD-L2, PDL2, B7DC
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
24Fdot10C12_PEDazzle594_CD273_082217
Human monocyte-derived dendritic cells were stained with anti-human CD273 (clone 24F.10C12) PE/Dazzle™ 594 (filled histogram) or PE/Dazzle™ 594 mouse IgG2a, κ isotype control (open histogram).
  • 24Fdot10C12_PEDazzle594_CD273_082217
    Human monocyte-derived dendritic cells were stained with anti-human CD273 (clone 24F.10C12) PE/Dazzle™ 594 (filled histogram) or PE/Dazzle™ 594 mouse IgG2a, κ isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
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329617 25 tests 137€
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329618 100 tests 306€
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Description

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PD-L2 cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Brown JA, et al. 2003. J. Immunol. 170:1257
  2. Rodig N, et al. 2003. Eur. J. Immunol. 33:3117
Product Citations
  1. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
RRID
AB_2716089 (BioLegend Cat. No. 329617)
AB_2716089 (BioLegend Cat. No. 329618)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 25 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor
PD-1
Cell Type
Dendritic cells, Macrophages
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
80380 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org
Go To Top Version: 0    Revision Date: 08/22/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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