- Clone
- 42D1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD85, ILT4, MIR10, Leukocyte Immunoglobulin-Like Receptor subfamily B member 2 (LILRB2), Leukocyte Immunoglobulin-like Receptor 2 (LIR-2)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![42D1_PEDazzle594_CD85d_Antibody_060418 42D1_PEDazzle594_CD85d_Antibody_060418](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/42D1_PEDazzle594_CD85d_Antibody_060418.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with CD85d (ILT4, clone 42D1) PE/Dazzle™ 594 (filled histogram) or Rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram) in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426103).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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338709 | 25 tests | 113€ | ||||
338710 | 100 tests | 300€ |
CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subset of ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while the inhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85d is a 95kD inhibitory receptor, also known as ILT4, LIR2, or MIR10. ILT4 is expressed on the surface of monocytes/macrophages, and dendritic cells. ILT4 acts as an inhibitory receptor through recruitment of SHP-1 and SHP-2 protein tyrosine phosphatases. The ligands of ILT4 are HLA-A, -B and nonclassical MHC-I molecule HLA-G1.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm. - Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications include: negatively modulate myelomonocytic cells signaling. Enhance the binding of HLA-G tetramer to monocytes.
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Application References
(PubMed link indicates BioLegend citation) -
- Colonna M, et al. 1998. J. Immunol. 160:3096
- Allan DS. 1999. J. Exp. Med. 189:1149
- RRID
-
AB_2734343 (BioLegend Cat. No. 338709)
AB_2734344 (BioLegend Cat. No. 338710)
Antigen Details
- Structure
- ILT/LIR family, Ig superfamily, 95kD
- Distribution
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Monocytes/macrophages, dendritic cells
- Ligand/Receptor
- MHC class I molecules (HLA-A, -B) and nonclassic class I molecule HLA-G1
- Cell Type
- Dendritic cells, Macrophages, Monocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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- Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
- Shiroishi M, et al. 2003. Proc. Natl. Acad. Sci. 100:8856
- Colonna M, et al. 1998. J. Immunol. 160:3096
- Lichterfeld M, et al. 2007. J. Exp. Med. 204:2813
- Gene ID
- 10288 View all products for this Gene ID
- UniProt
- View information about CD85d on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD85d Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD85d (ILT4) | 42D1 | FC |
APC anti-human CD85d (ILT4) | 42D1 | FC |
PE/Dazzle™ 594 anti-human CD85d (ILT4) | 42D1 | FC |
PE/Cyanine7 anti-human CD85d (ILT4) | 42D1 | FC |
Ultra-LEAF™ Purified anti-human CD85d (ILT4) | 42D1 | FC,FA |
TotalSeq™-B1249 anti-human CD85d (ILT4) | 42D1 | PG |
TotalSeq™-D1249 anti-human CD85d (ILT4) | 42D1 | PG |
TotalSeq™-C1249 anti-human CD85d (ILT4) | 42D1 | PG |
TotalSeq™-A1249 anti-human CD85d (ILT4) | 42D1 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD85d (ILT4)
Human peripheral blood monocytes stained with 42D1 PE and CD... -
APC anti-human CD85d (ILT4)
Human peripheral blood monocytes were stained with CD85d (cl... -
PE/Dazzle™ 594 anti-human CD85d (ILT4)
Human peripheral blood monocytes were stained with CD85d (IL... -
PE/Cyanine7 anti-human CD85d (ILT4)
Human peripheral blood monocytes were stained with CD85d (IL... -
Ultra-LEAF™ Purified anti-human CD85d (ILT4)
Human peripheral blood monocytes stained with LEAF™ purified... -
TotalSeq™-B1249 anti-human CD85d (ILT4)
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TotalSeq™-D1249 anti-human CD85d (ILT4)
-
TotalSeq™-C1249 anti-human CD85d (ILT4)
-
TotalSeq™-A1249 anti-human CD85d (ILT4)
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