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PE/Dazzle™ 594 anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
1G9 (See other available formats)
Regulatory Status
RUO
Other Names
VSIG9
Isotype
Mouse IgG1, κ
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Product Citations
publications
1G9_PEDZL594_TIGIT_Antibody_FC_1_120115
C57BL/6 Mouse splenocytes were stimulated with Con A and IL-2 for three days, and then stained with CD3 FITC (Clone 145-2C11) and TIGIT (Clone 1G9) PE/Dazzle™ 594 (Top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (Bottom).
  • 1G9_PEDZL594_TIGIT_Antibody_FC_1_120115
    C57BL/6 Mouse splenocytes were stimulated with Con A and IL-2 for three days, and then stained with CD3 FITC (Clone 145-2C11) and TIGIT (Clone 1G9) PE/Dazzle™ 594 (Top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (Bottom).
  • 1G9_PEDZL594_TIGIT_Antibody_FC_2_120115
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability Save
142109 25 µg 118€
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142110 100 µg 306€
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and transmembrane domain-containing protein 3 (Vstm3), is a 26 kD, type I transmembrane protein and member of the CD28 family. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. Its binding partners include CD155 (PVR) and CD112 (PVRL2). TIGIT is a negative regulator of NK and T cell activation. Engagement of TIGIT by dendritic cells results in their differentiation into a tolerogenic phenotype, with an increased secretion of IL-10 and a diminished production of IL-12. Mice deficient for TIGIT are more susceptible to autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Joller N, et al. 2010. J. Immunol. 186:1338.
Product Citations
  1. Campisi L, et al. 2022. Nature. 606:945. PubMed
  2. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  3. del Rio ML, et al. 2023. Front Immunol. 14:1113858. PubMed
  4. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  5. Hao C, et al. 2021. Frontiers in Cell and Developmental Biology. 9:695015. PubMed
  6. Beltra JC, et al. 2020. Immunity. 52(5):825-841. PubMed
  7. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  8. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
RRID
AB_2566572 (BioLegend Cat. No. 142109)
AB_2566572 (BioLegend Cat. No. 142110)

Antigen Details

Structure
Type I transmembrane protein, 26 kD, member of the CD28 family. Contains 1 Ig-like domain and 1 cytoplasmic ITIM motif.
Distribution

Activated T cells, follicular T helper cells, NK, memory T cells, Tregs.

Function
Negative regulator of T cell activation and proliferation. Induction of tolerogenic dendritic cells.
Ligand/Receptor
CD155 (PVR) and CD112 (PVRL2).
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Levin SD, et al. 2011. Eur. J. Immunol. 41:902.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Stanietsky N, et al. 2009. P. Natl. Acad. Sci. USA 106:17858.

Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/19/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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