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PerCP/Cyanine5.5 anti-mouse CD183 (CXCR3) Antibody

Pricing & Availability
Clone
CXCR3-173 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype
Armenian Hamster IgG
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Product Citations
publications
CXCR3-173_PerCPCyanine55_CD183_Antibody_080818
C57BL/6 splenocytes were stained with CD3e APC and CD183 PerCP/Cyanine5.5 (clone CXCR3-173) (left), or PerCP/Cyanine5.5 Armenian Hamster IgG isotype control (right).
  • CXCR3-173_PerCPCyanine55_CD183_Antibody_080818
    C57BL/6 splenocytes were stained with CD3e APC and CD183 PerCP/Cyanine5.5 (clone CXCR3-173) (left), or PerCP/Cyanine5.5 Armenian Hamster IgG isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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126513 25 µg 118€
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126514 100 µg 287€
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Description

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse N-terminus of CXCR3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Krug A, et al. 2002. J. Immunol. 169:6079.
  2. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
  3. Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
  4. Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
  5. Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
  6. Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
  7. Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations
  1. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  2. Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed
  3. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  4. Files M, et al. 2022. NPJ Vaccines. 7:48. PubMed
  5. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  6. Ferreira CP, et al. 2017. Front Immunol. 1.229861111. PubMed
  7. Zhang L, et al. 2021. Clin Transl Med. 11:e464. PubMed
  8. Jeong M, et al. 2022. JCI Insight. 7:. PubMed
  9. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  10. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
  11. Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
RRID
AB_1186017 (BioLegend Cat. No. 126513)
AB_1186017 (BioLegend Cat. No. 126514)

Antigen Details

Distribution

T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors

Function
Essential in T cell recruitment to sites of inflammation
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID
12766 View all products for this Gene ID
UniProt
View information about CD183 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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