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PerCP/Cyanine5.5 anti-mouse CD2 Antibody

Pricing & Availability
Clone
RM2-5 (See other available formats)
Regulatory Status
RUO
Other Names
LFA-2, T11, Ly-37, SRBC-R
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
RM2-5_PerCPCy5.5_CD2_Antibody_FC_042414
C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • RM2-5_PerCPCy5.5_CD2_Antibody_FC_042414
    C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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100115 25 µg 100€
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100116 100 µg 259€
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Description

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
  2. Ayroldi E, et al. 1997. Blood 89:3717. (Block)
  3. Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
  4. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
Product Citations
  1. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  2. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  3. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  4. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
RRID
AB_2563501 (BioLegend Cat. No. 100115)
AB_2563501 (BioLegend Cat. No. 100116)

Antigen Details

Structure
Ig superfamily, 45-58 kD
Distribution

T cells, thymocytes, NK cells, B cells

Function
T cell activation, adhesion
Ligand/Receptor
CD48, lck
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.

Gene ID
12481 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 04/29/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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