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PerCP/Cyanine5.5 anti-mouse CD5 Antibody

Pricing & Availability
Clone
53-7.3 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-7.3_PerCpCy5.5_CD5_Antibody_FC_041714
C57BL/6 mouse splenocytes were stained with CD5 (clone 53-7.3) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a PerCP/Cyanine5.5 isotype control (open histogram).
  • 53-7.3_PerCpCy5.5_CD5_Antibody_FC_041714
    C57BL/6 mouse splenocytes were stained with CD5 (clone 53-7.3) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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100623 25 µg 118€
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100624 100 µg 278€
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Description

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
  3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  3. Català C, et al. 2022. iScience. 25:105078. PubMed
  4. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  5. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  6. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  7. Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
  8. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  9. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  10. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  11. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  12. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  13. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  14. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  15. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  16. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  17. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  18. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  19. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  20. Goc J, et al. 2021. Cell. 184:5015. PubMed
RRID
AB_2563432 (BioLegend Cat. No. 100623)
AB_2563432 (BioLegend Cat. No. 100624)

Antigen Details

Structure
Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution

Thymocytes, T cells, B-1 cells

Function
Negative regulator of T-B cell interaction
Ligand/Receptor
CD72, gp35-37
Cell Type
B cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.

Gene ID
12507 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 05/14/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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