PerCP/Cyanine5.5 anti-mouse IL-9 Antibody anti-IL-9

Pricing & Availability

Clone: RM9A4 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype: Rat IgG1, κ
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Product Citations: publications

RM9A4_PerCPCyanine55_IL-9_Antibody_1_041124 — Enriched C57BL/6 mouse CD4+ T cells were stimulated with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-β for three days, then re-stimulated with PdBU and ionomycin (in the presence of brefeldin A) for 5 hours, fixed, permeabilized, and then stained with CD4 FITC and IL-9 (clone RM9A4) PerCP/Cyanine5.5 (left) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (right).

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514111

25 µg

141€

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514112

100 µg

329€

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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full length mouse IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application Notes: See the protocol for Th9 polarization of mouse splenocytes.
RRID: AB_2562729 (BioLegend Cat. No. 514111)
AB_2562729 (BioLegend Cat. No. 514112)

Antigen Details

Structure: Cytokine; 30-40 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated T_H2 lymphocytes
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 16198 View all products for this Gene ID
UniProt: View information about IL-9 on UniProt.org

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Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All IL-9 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse IL-9	RM9A4	ICFC
Purified anti-mouse IL-9	RM9A4	ELISA
APC anti-mouse IL-9	RM9A4	ICFC
Alexa Fluor® 647 anti-mouse IL-9	RM9A4	ICFC
Brilliant Violet 421™ anti-mouse IL-9	RM9A4	ICFC
PerCP/Cyanine5.5 anti-mouse IL-9	RM9A4	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

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