PerCP/Fire™ 806 anti-human CD123 Antibody, CD123, S18016C

Pricing & Availability

Clone: S18016C (See other available formats)
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ
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Product Citations: publications

S18016C_PerCPFire806_CD123_1_091124 — Human peripheral blood lymphocytes were stained with anti-human CD45RO (clone UCHL1) Spark Violet™ 423 and anti-human CD123 (clone S18016C) PerCP/Fire™ 806 (left) or mouse IgG1, κ PerCP/Fire™ 806 isotype control (right).

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396615

25 tests

148€

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396616

100 tests

316€

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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hu CD123 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 806 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Fire™ 806 has a maximum excitation of 478 nm and a maximum emission of 806 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3662296 (BioLegend Cat. No. 396615)
AB_3662296 (BioLegend Cat. No. 396616)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563 View all products for this Gene ID
UniProt: View information about CD123 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD123 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD123	S18016C	FC
Purified anti-human CD123	S18016C	FC
PE/Dazzle™ 594 anti-human CD123	S18016C	FC
APC anti-human CD123	S18016C	FC
PerCP/Cyanine5.5 anti-human CD123	S18016C	FC
PE/Cyanine7 anti-human CD123	S18016C	FC
PerCP/Fire™ 806 anti-human CD123	S18016C	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/03/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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