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PhosphoPair mTOR (Ser2448) Antibody Set

Pricing & Availability
Clone
6H9B10; A17024A;
Regulatory Status
RUO
Other Names
Mechanistic Target of Rapamycin Kinase, FRAP1, RAFT, FKBP-Rapamycin Associated Protein
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Product Citations
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PhosphoPair_mTOR_Antibody_Set_081120.png
Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-mTOR Phospho (Ser2448) antibody (clone A17024A), for 2 hours at room temperature. To confirm phospho-specificity of A17024A, a duplicate membrane was pre-treated with lambda protein phosphatase prior to incubation with A17024A. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal mTOR loading was confirmed by probing membranes with purified anti- mTOR antibody (clone 6H9B10) at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
  • PhosphoPair_mTOR_Antibody_Set_081120.png
    Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-mTOR Phospho (Ser2448) antibody (clone A17024A), for 2 hours at room temperature. To confirm phospho-specificity of A17024A, a duplicate membrane was pre-treated with lambda protein phosphatase prior to incubation with A17024A. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal mTOR loading was confirmed by probing membranes with purified anti- mTOR antibody (clone 6H9B10) at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
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Description

mTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, hormones, growth factors, and stress. mTOR is found as a component of two functionally distinct oligomeric complexes, mTORC1 and mTORC2 (mTOR complexes 1 and 2). mTORC1 coordinates nutrient availability with cell growth and proliferation through phosphorylation of upstream regulators of proliferation and ribosomal biogenesis including eIF4BP1, RPS6KB1 and RPS6KB2. Additionally, mTORC1 controls autophagy through negative regulation of an autophagy-promoting kinase complex comprised of ULK1, ATG13, and FIP200. mTORC2 regulates proliferation and metabolism via phosphorylation of IG-1R, InsR, and Akt, and also plays a role in cytoskeletal architecture and reorganization through modification of Rac1.

The mTOR subunit of mTORC1 and mTORC2 is phosphorylated at Ser2448 in a PI3/Akt-signaling-dependent manner; this post-translational modification is associated with increased mTORC activity.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Product Application Clone Size Reactivity Isotype
Purified anti-mTOR Antibody WB 6H9B10 25 μg Human, Mouse Mouse IgG1, κ
Purified mTOR Phospho (Ser2448) Antibody WB, ICC A17024B 25 μg Human, Mouse Mouse IgG1, κ


* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Verified Reactivity
Human, Mouse
Immunogen
Please refer to individual product datasheets for immunogen information
Formulation
Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation
The antibodies were purified by affinity chromatography.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Clone 6H9B10: 0.25 - 1.0 µg/mL
Clone A17024A: 0.1 - 1.0 µg/mL

It is recommended that the reagent be titrated for optimal performance for each application.

For recommended usage information for additional applications, please refer to the datasheet of the individual products.

Antigen Details

Structure
mTOR is a 2,549 amino acid protein with a predicted molecular weight of ~289 kD.
Distribution

Ubiquitously expressed/cytosolic, lysosomal, and nuclear cellular localization

Function
Regulates cell proliferation and metabolism
Antigen References
  1. Brown EJ, et al. 1994. Nature. 369:756-8.
  2. Dennis P, et al. 2001. Science. 294:1102.
  3. Fingar D, et al. 2002. Genes Dev. 16:1472.
  4. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.
Gene ID
2475 View all products for this Gene ID
UniProt
View information about mTOR on UniProt.org
Go To Top Version: 1    Revision Date: 08/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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