Loading...

Purified anti-Pan AKT Antibody

Pricing & Availability
Clone
A21001A (See other available formats)
Regulatory Status
RUO
Other Names
Protein kinase B, PKB, RAC-Alpha, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine-protein kinase
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
A. A21001A_PURE_Pan-AKT_WB_050725
Whole cell lysates (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Pan AKT (clone A21001A). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A21001A_PURE_Pan-AKT_WB_050725
    Whole cell lysates (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Pan AKT (clone A21001A). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A21001A_PURE_Pan-AKT_ICC_050725
    MCF-7 cells (high expression control, top panels) and HEK293 cells (medium-low expression control, bottom, panels) were fixed and permeabilized with cold methanol, blocked with 5% FBS, and then stained with Purified anti-Pan AKT (clone A21001A) (middle and right panels) or Purified mouse IgG2b, κ Isotype Control (Cat. No 401201) (left panels), followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801) and merged in the left and middle panel. Images were captured on a Revvity Operetta CLS™ High Content Analysis System with a with a 63X objective. Scale bar: 50 µm.
  • C. A21001A_PURE_Pan-AKT_IHC-P_050725
    IHC staining with anti-Pan AKT (clone A21001A) was performed on formalin-fixed paraffin-embedded human testis tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated without (panel A) or with anti-Pan AKT (clone A21001A) (panels B and C), followed by incubation with Alexa Fluor® 647 Goat anti-mouse (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801) (merged in panels A and C). Scale Bar: 50 µm
  • d. A21001A_PURE_Pan-AKT_ICFC_050725
    MCF-7 cells (positive cell line, filled histogram) and HEK293 cells (medium-low-expressing cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-Pan AKT (clone A21001A), or Purified mouse IgG2b, κ Isotype Control (open histogram, dashed line) (Cat No. 401201), followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322).
Cat # Size Price Quantity Check Availability Save
646151 25 µg 136€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
646152 100 µg 372€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The AKT family of proteins, also known as Protein Kinase B (PKB), consists of three isoforms: AKT1, AKT2, and AKT3. These serine/threonine kinases are pivotal in regulating various cellular processes, including glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. AKT1 is primarily involved in cellular survival pathways and is a key player in inhibiting apoptosis, promoting cell growth, and protein synthesis. AKT2 is crucial in the insulin signaling pathway, facilitating glucose transport and maintaining glucose homeostasis. AKT3 is predominantly expressed in the brain and is essential for brain development. Dysregulation of AKT signaling is associated with numerous diseases, including cancer, diabetes, and neurological disorders such as Alzheimer's disease. Overexpression or mutations in AKT genes can lead to uncontrolled cell growth and survival, contributing to tumorigenesis in various cancers. Additionally, aberrant AKT signaling is implicated in insulin resistance and type 2 diabetes, as well as neurodegenerative diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide for human AKT
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody detects all 3 isoforms of AKT protein kinases (AKT1, AKT2, AKT3).

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using either True-Phos™ Perm Buffer (Cat. No. 425401), or Cyto-Fast Fix/Perm Buffer set (Cat. No. 426803)

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using either PFA-Triton X-100, or Methanol only.

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

Antigen Details

Structure
AKT Molecular Weight: ~ 56 kDa
Distribution

Nucleoplasm and cytoplasm

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immuno-Oncology
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Krzeslak A, et al. 2011. Endocrine Pathology. 22:206.
  2. Jian K, et al. 2003. Blood. 101:236.
  3. Manning BD, et al. 2017. Cell. 169:381.
  4. Song M, et al. 2019. Cancer Res. 79:1019.
  5. Revathidevi S, et al. 2019. Semin Cancer Biol. 59:80.
Gene ID
207 View all products for this Gene ID 208 View all products for this Gene ID 1000 View all products for this Gene ID
UniProt
View information about AKT on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All AKT Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Pan AKT A21001A WB,ICC,ICFC,IHC-P
Go To Top Version: 1    Revision Date: 05/07/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account