Purified anti-AMIGO-1 Antibody (Previously Covance catalog# MMS-5268)

Pricing & Availability
Clone
L86A/37 (See other available formats)
Regulatory Status
RUO
Other Names
Amphoterin-induced protein 1, alivin-2, amphoterin-induced gene and ORF
Previously
Covance Catalog# MMS-5268
Isotype
Mouse IgG2b, κ
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Product Citations
publications
L86A37_Pure_AMIGO1_Antibody_1_011018
IHC staining of purified anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L86A37_Pure_AMIGO1_Antibody_1_011018
    IHC staining of purified anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L86A37_Pure_AMIGO1_Antibody_2_011018
    IHC staining of purified anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L86A37_Pure_AMIGO1_Antibody_3_011018
    IHC staining of purified anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L86A37_Pure_AMIGO1_Antibody_4_011018
    Western blot of purified anti-AMIGO-1 antibody (clone L86A/37). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • L86A37_Pure_AMIGO1_Antibody_5_011018
    IHC staining of purified anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • L86A37_Pure_AMIGO1_Antibody_6_011018
    IHC staining of purified anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
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822101 100 µL 132€
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Description

Amphoterin-induced protein 1 (AMIGO-1) is a transmembrane protein containing six LRR domains and one Ig domain and is highly expressed in the mammalian nervous system. It promotes growth and fasciculation of neurites from cultured hippocampal neurons and may be involved in fasciculation as well as myelination of developing neural axons. AMIGO-1 may have a role in regeneration as well as neural plasticity in the adult nervous system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Mandikian D, et al. 2014. Comp Neurol. 522(15): 3555. (IHC-P)
RRID
AB_2564856 (BioLegend Cat. No. 822101)

Antigen Details

Structure
Amigo-1 has an apparent molecular weight ranging from 60-80 kD.
Distribution

Tissue distribution: Nervous system
Cellular distribution: Plasma membrane

Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Adhesion Molecules
Antigen References

1. Chen Y, et al. 2006. Brain Res Rev. 51(2):265.

Gene ID
57463 View all products for this Gene ID
UniProt
View information about AMIGO-1 on UniProt.org
Go To Top Version: 6    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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