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Purified anti-Beta-Arrestin 1 Antibody

Pricing & Availability
Clone
W20187A (See other available formats)
Regulatory Status
RUO
Other Names
Beta-Arrestin-1 (ARBB1), Arrestin beta-1, Non-visual arrestin-2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W20187A_PURE_-Beta-Arrestin-1_WB_012225
Whole cell extracts (15 µg total protein per lane) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Beta-Arrestin 1 (clone W20187A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W20187A_PURE_-Beta-Arrestin-1_WB_012225
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Beta-Arrestin 1 (clone W20187A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W20187A_PURE_-Beta-Arrestin-1_IP_012225
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnightwith Purified rat IgG2b, κ Isotype Control (Cat. No. 400602) or Purified anti-Beta-Arrestin 1 (clone W20187A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of ARRB1. Lane M: Molecular weight marker
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652951 25 µg 144€
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652952 100 µg 344€
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Description

Beta-arrestin 1, a member of the arrestin family, is a crucial regulatory protein involved in G protein-coupled receptor (GPCR) signaling, modulating GPCR activity following receptor activation. Arrestins bind directly to phosphorylated GPCRs and preclude their interaction with other proteins, thus inhibiting further signaling and preventing overstimulation. Furthermore, binding of arrestins like Beta-arrestin 1 can result in receptor internalization, leading potentially to ligand-receptor decoupling and receptor recycling, or to receptor degradation and a reduction in the available receptors in the cell. Thus Beta-arrestin 1 regulates a wide variety of physiological processes including inflammation, metabolism, and cell migration, highlighting its importance in cellular communication and homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant fragment of human Beta-Arrestin 1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 0.25 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not cross react with mouse Beta-Arrestin 1 in Western Blot.

This product is not recommended for use in immunocytochemistry (ICC) or immunohistochemistry in formalin-fixed, paraffin-embedded tissues (IHC-P).

Antigen Details

Structure
Beta-arrestin 1 is a 418 amino acid protein with a predicted molecular weight of 47.1 kD.
Distribution

Cytoplasm, plasma membrane, and in clathrin-coated pits upon receptor internalization

Function
GPCR regulation
Interaction
Forms dimers and tetramers. Binds to phosphorylated GPCRs such as ADRB2, rhodopsin and CHRM2.
Cell Type
Neurons
Biology Area
Cardiovascular Biology, Cell Biology, Neuroscience, Signal Transduction
Molecular Family
Adaptor Proteins
Antigen References
  1. Wess J, et al. 2023. Pharmacol Rev. 75:854-884.  
  2. Gurevich VV and Gurevich EV. 2023. Curr Protoc. 3:e821. 
  3. Latorraca NR, et al. 2021. Cell. 183:1813-1825.
Gene ID
408 View all products for this Gene ID
UniProt
View information about Beta-Arrestin 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/22/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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