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Purified anti-β-Dystroglycan Antibody

Pricing & Availability
Clone
7D11 (See other available formats)
Regulatory Status
RUO
Other Names
Beta-dystroglycan, Beta-DG
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_7D11_PURE_betaDystro_Antibody_5_121918.png
Western blot of purified anti-β-Dystroglycan antibody (clone 7D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_7D11_PURE_betaDystro_Antibody_5_121918.png
    Western blot of purified anti-β-Dystroglycan antibody (clone 7D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 7D11_PURE_betaDystro_Antibody_1_082417
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 7D11_PURE_betaDystro_Antibody_2_121918_updated.png
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 7D11_PURE_betaDystro_Antibody_3_121918.png
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 7D11_PURE_betaDystro_Antibody_4_121918.png
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded mouse heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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850201 25 µg 76€
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850202 100 µg 193€
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Description

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and Utrophin scaffolds axin to the cytoskeleton. β-dystroglycan also has been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Praissman JL, et al. 2016. Elife. E14473 (ICC)
  2. Vasquez-Limeta A, et al. 2014. PLoS One. 9(3):e90629 (WB)
  3. Martinez-Vieyra IA, et al. 2013. biochim Biophys Acta. 1833(3):698 (ICC, WB)
  4. Beedle AM, et al. 2012. J Clin Invest. 122(9):3330 (IHC-F, WB)
  5. Hu H, et al. 2011. Neurosci Lett. 489(1): 10 (WB)
Product Citations
  1. Dai J, et al. 2022. Nat Cancer. 3:25. PubMed
RRID
AB_2715870 (BioLegend Cat. No. 850201)
AB_2715870 (BioLegend Cat. No. 850202)

Antigen Details

Structure
Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
Distribution

Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS.
Cellular distribution: nucleus, plasma membrane, and cytoskeleton.

Function
β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Interaction
Interacts with Dystrophin, Utrophin and other cytoskeletal components such as axin.
Ligand/Receptor
Receptor for Dystrophin and Utrophin
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Murphy S, et al. 2015. Comput Struct Biotechnol J. 14:20-7
2. Moore CJ, et al. 2012. Neuromuscul Disord. 22(11): 959

Gene ID
1605 View all products for this Gene ID
UniProt
View information about beta-Dystroglycan on UniProt.org
Go To Top Version: 3    Revision Date: 12/19/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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