- Clone
- A22009C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A.A22009C_PURE_C-Fos_WB_021324 A.A22009C_PURE_C-Fos_WB_021324](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A22009C_PURE_C-Fos_WB_021324.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from HeLa cells untreated or treated with the Cell Activation Cocktail (without Brefeldin A) (Cat No. 423301) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then untreated (left blot) or LPP-treated (right blot). The blots were probed with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
HeLa cells untreated (top panels), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours (bottom panels) were fixed and permeabilized with 2% PFA and ice-cold methanol, and then intracellularly stained with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) (right panels) or Purified mouse IgG2b, κ Isotype Control (Cat. No. 402201) (left panels) followed by Alexa Fluor® 647 Goat anti-Mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (clone C-11) (Cat. No. 628608) (green). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm -
HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) or Purified mouse IgG2b, κ Isotype Control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 402201) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). -
IHC staining of Purified anti-c-Fos Phospho (Ser32) on formalin-fixed paraffin-embedded human brain cortex tissue was performed following antigen retrieval with Citrate buffer (Cat. No. 420902). The tissue sections were incubated with Alexa Fluor® 647 Goat anti-mouse IgG only (Cat. No. 405322) (panel A) or with Purified anti-c-Fos Phospho (Ser32) (clone A22009C) (panel B) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG. Nuclei were counterstained with DAPI (Cat. No. 422801) Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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631251 | 25 µg | 144€ | ||||
631252 | 100 µg | 364€ |
The proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases. In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human c-Fos phosphorylated at Ser32.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ICFC, IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.012 - 0.1 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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For ICFC use we recommend cell fixation/permeabilization with True-Phos™ Perm buffer.
For use of this antibody in immunohistochemistry on formalin-fixed paraffinembedded tissues (IHC-P), we recommend antigen retrieval with Citrate buffer (Cat. No. 420902). However, the antibody also performs well with antigen retrieval with Tris-EDTA (Cat. No. 422703). - RRID
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AB_3097545 (BioLegend Cat. No. 631251)
AB_3097545 (BioLegend Cat. No. 631252)
Antigen Details
- Structure
- c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.
- Distribution
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Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus.
- Function
- c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.
- Interaction
- c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.
- Ligand/Receptor
- As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors
- Molecular Family
- Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors
- Antigen References
-
- Sasaki T, et al. 2006. Mol Cell. 24:63-75.
- Huang K, et al. 2014. Cell Signal. 26:629-38.
- Qu X, et al. 2019. Cancer Sci. 110:3183-3196.
- Guo JC, et al. 2015. PLoS One. 10:e0120332.
- Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.
- Gene ID
- 2353 View all products for this Gene ID
- UniProt
- View information about c-Fos on UniProt.org
Other Formats
View All c-Fos Phospho Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-c-Fos Phospho (Ser32) | A22009C | WB,ICC,ICFC |
PE anti-c-Fos Phospho (Ser32) | A22009C | ICFC |
Alexa Fluor® 488 anti-c-Fos Phospho (Ser32) | A22009C | ICFC |
Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for Flow Cytometry) | A22009C | ICFC |
Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for IHC and ICC) | A22009C | IHC-P,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-c-Fos Phospho (Ser32)
Whole cell extracts (15 µg total protein) from HeLa cells un... HeLa cells untreated (top panels), or treated with Cell Acti... HeLa cells untreated (negative control, open histogram), or ... IHC staining of Purified anti-c-Fos Phospho (Ser32) on forma... -
PE anti-c-Fos Phospho (Ser32)
HeLa cells untreated (negative control, open histogram), or ... HeLa cells treated with Cell Activation Cocktail (without Br... -
Alexa Fluor® 488 anti-c-Fos Phospho (Ser32)
HeLa cells untreated (negative control, open histogram), or ... HeLa cells treated with Cell Activation Cocktail (without Br... -
Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for Flow Cytometry)
HeLa cells untreated (negative control, open histogram), or ... -
Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for IHC and ICC)
IHC staining with Alexa Fluor® 647 anti-c-Fos Phospho (Ser32... HeLa cells untreated or treated with Cell Activation Cocktai...
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