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Purified anti-Clathrin Heavy Chain (CLTCL2) Antibody (Previously Covance catalog# MMS-427P)

Pricing & Availability
Clone
TD.1 (See other available formats)
Regulatory Status
RUO
Other Names
Clathrin heavy chain 1, clathrin, heavy polypeptide-like 2, clathrin heavy chain on chromosome 17
Previously
Covance Catalog# MMS-427P
Isotype
Mouse IgG1, κ
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Product Citations
publications
TD1_Pure_CLTCL2_Antibody_1_053118
Western blot of purified anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • TD1_Pure_CLTCL2_Antibody_1_053118
    Western blot of purified anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • TD1_Pure_CLTCL2_Antibody_2_051519
    Western blot of purified anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 436302). Enhanced chemiluminescence was used as the detection system.
  • TD1_Pure_CLTCL2_Antibody_3_100319
    ICC staining of purified anti-Clathrin Heavy Chain (CLTCL2) antibody (clone TD.1) on Drosophila S2 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 10 µm
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813901 100 µL 231€
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Description

Clathrin consists of three heavy chains (192 kD) and three light chains (LCa or LCb, 25-29 kD) and self assembles into a polyhedral cytoskeletal element. The self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy chain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not react with native clathrin in immunoprecipitation.

Reactivity to Drosophila was only verified with the purified format.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 5 -10 µg/ml in human, mouse, and rat and 1-10 µg/ml in Drosophila.

Application References

(PubMed link indicates BioLegend citation)
  1. Hood FE and Royle SJ. 2009. J Cell Sci. 122:2185-2190. (ICC) PubMed
  2. Jiang R, et al. 2000. J Biol Chem 275(12):8439-8447. (IP)
Product Citations
  1. Royle F 2009. J Cell Sci. 122:2185-2190. PubMed
  2. Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed
RRID
AB_2564779 (BioLegend Cat. No. 813901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
1213 View all products for this Gene ID
UniProt
View information about Clathrin Heavy Chain on UniProt.org
Go To Top Version: 6    Revision Date: 03/12/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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