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Purified anti-CUL3 Antibody

Pricing & Availability
Clone
W21054D (See other available formats)
Regulatory Status
RUO
Other Names
Cullin-3, CUL-3, Cullin3, PHA2E, KIAA0617
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W21054D_PURE_CUL3_WB_102824
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CUL3 (clone W21054D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W21054D_PURE_CUL3_WB_102824
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CUL3 (clone W21054D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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642151 25 µg 120€
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642152 100 µg 332€
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Description

CUL3 (Cullin-3) is a key component of the ubiquitin-proteasome system (UPS), an essential pathway for regulating protein degradation in human cells. It functions as part of an E3 ubiquitin ligase complex, which tags specific proteins with ubiquitin molecules, marking them for degradation by the proteasome. CUL3 is a member of the Cullin family of proteins, which provide a structural scaffold for the assembly of multi-subunit ubiquitin ligases, termed Cullin-RING ligases (CRLs). These ligases play a central role in maintaining protein homeostasis by controlling the turnover of numerous regulatory proteins involved in critical cellular processes such as cell cycle progression, signal transduction, transcription, and stress responses.

CUL3 forms a complex with a RING-domain protein, RBX1, which recruits the E2 ubiquitin-conjugating enzyme, and various substrate adaptors known as BTB domain-containing proteins. The BTB proteins determine substrate specificity, allowing CUL3 to target a wide range of proteins for degradation. This ability to selectively degrade proteins makes CUL3 a crucial regulator of several physiological and pathological processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human CUL3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W21054D), is not recommended for use in either immunocytochemistry (ICC) or immunohistochemistry (IHC-P).  

Antigen Details

Structure
CUL3 is a 768 amino acid length protein with a predicted molecular weight of 88.9 kD.
Distribution

Located in nucleus, Golgi apparatus, and cytoplasm

Function
Mediates the ubiquitination and subsequent proteasomal degradation of target proteins
Interaction
A component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes
Biology Area
Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family
Adaptor Proteins, Enzymes and Regulators
Antigen References
  1. Canning P, et al, 2013, J Biol Chem.288:7803-7814.
  2. McGourty C, et al. 2016. Cell. 167:525-538.e14.
  3. Dong Z, et al. 2019. Neuron. 105:475-490.e6.
Gene ID
8452 View all products for this Gene ID
UniProt
View information about CUL3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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