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Purified anti-DDX17 (p82) Antibody

Pricing & Availability
Clone
1B5B09 (See other available formats)
Regulatory Status
RUO
Other Names
Probable ATP-dependent RNA helicase DDX17, DEAD box protein p82, RNA-dependent helicase p82
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1B5B09_PURE_DDX17(p82)_Antibody_1_WB_071916
Total lysates (15 µg protein) from HeLa, U87-MG (human) and NIH3T3 (mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 (0.25 µg/ml) purified anti-DDX17 antibody, clone 1B5B09. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1B5B09_PURE_DDX17(p82)_Antibody_1_WB_071916
    Total lysates (15 µg protein) from HeLa, U87-MG (human) and NIH3T3 (mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 (0.25 µg/ml) purified anti-DDX17 antibody, clone 1B5B09. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1B5B09_PURE_DDX17(p82)_Antibody_2_IF_092116
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-DDX17 (p82) Antibody (clone 1B5B09) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 1B5B09_PURE_DDX17(p82)_Antibody_3_IP_071916
    Immunoprecipitation of DDX17 from Hela cell extracts. Lane 1 is 15% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-DDX17 antibody (clone 1B5B09, lane 4). Western blot was performed using anti-DDX17 antibody (clone 1B5B09).
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657202 100 µg 259€
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Description

DDX17 is an ATP-dependent RNA helicase, belonging to the DEAD (Asp-Glu-Ala-Asp) box superfamily and highly related to the other key family member DDX5. The RNA helicase activity of DDX17 has been implicated in the regulation of RNA metabolism, such as RNA splicing, RNA editing, RNA degradation, and modulating secondary RNA structure. DDX17 can act as a transcriptional co-activator for the estrogen receptor ERα. It is also involved in muscle cell differentiation by co-activating MyoD. DDX17 regulates histone modification through interacting with the acetyltransferases p300, CBP, and PCAF, and the histone deacetylase HDAC1. DDX17 deficient mice exhibit severe developmental problems and die shortly after birth.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DDX17 recombinant protein (1-107 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 -10 μg per ml. For immunocytochemistry, the suggested usage is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody, clone 1B5B09, detects only human DDX17 isoform-1 (p82) by Western blotting. Clone 7G6B09 detects both human DDX17 isoform-1 (p82) and isoform-2 (p72) by Western blotting.

RRID
AB_2562550 (BioLegend Cat. No. 657202)

Antigen Details

Structure
729 amino acids, predicted molecular weight of 80 kD. Contains a helicase domain, an ATP-binding domain, and a transactivation domain.
Distribution

Nucleus

Function
DDX17 is a member of the DEAD box superfamily of RNA helicases, involved in RNA metabolism and transcriptional regulation.
Interaction
DDX17 interacts with DDX5, RNA polymerase II, CBP, p300, PCAF, NCOA1, NCOA2, NCOA3, ESR1, TP53, and HDAC1.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Antigen References

1. Janknecht R. 2010. Am. J. Transl. Res. 2:223.
2. Wortham NC, et al. 2009. Oncogene. 28:4053.
3. Shin S, et al. 2007. Cancer Res. 67:7572.
4. Caretti G, et al. 2007. Cell Cycle. 6:1172.
5. Hönig A, et al. 2002. Mol. Cell Biol. 22:5698.
6. Lamm GM, et al. 1996. Nucleic Acids Res. 24:3739.

Gene ID
10521 View all products for this Gene ID
UniProt
View information about DDX17 on UniProt.org
Go To Top Version: 6    Revision Date: 11/30/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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