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Purified anti-DDX6 Antibody

Pricing & Availability
Clone
11G10B39 (See other available formats)
Regulatory Status
RUO
Other Names
Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
11G10B39_PURE_DDX6_Antibody_1_WB_121217
Whole cell lysates (15 µg protein) from Jurkat, HeLa, and NIH/3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-DDX6 antibody, clone 11G10B39. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot HRP anti-β-actin antibody (1:5000 dilution, Cat. No. 643807) was used as a loading control (lower).  Lane M: Molecular weight ladder.
  • 11G10B39_PURE_DDX6_Antibody_1_WB_121217
    Whole cell lysates (15 µg protein) from Jurkat, HeLa, and NIH/3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-DDX6 antibody, clone 11G10B39. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot HRP anti-β-actin antibody (1:5000 dilution, Cat. No. 643807) was used as a loading control (lower).  Lane M: Molecular weight ladder.
  • 11G10B39_PURE_DDX6_Antibody_2_WB_121217
    Whole cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with the indicated concentration of anti-DDX6 antibody, clone 11G10B39. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot HRP anti-β-actin antibody (1:5000 dilution, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • 11G10B39_Purified_DDX6_Antibody_3_010821
    A431 cells were stained with purified anti-DDX6 clone (11G10B39) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
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674402 100 µg 231€
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Description

DDX6, also known as p54/RCK, is a member of the DEAD (Asp-Glu-Ala-Asp)-box RNA helicase family. DDX6 has been shown to localize to, and is required for the assembly of, processing bodies (P-bodies), which are cytoplasmic foci that consist of proteins involved in mRNA storage, processing, and turnover. DDX6 has ATP hydrolysis and RNA helicase activities, which are involved in secondary structure modulation and the degradation of mRNA. Interacting with key components of miRNA-induced silencing complexes, DDX6 participates in translational repression, as well. DDX6 is overexpressed in some colorectal tumor cells and can act as a proto-oncogene.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DDX6 recombinant protein (1-126 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.25 µg/mL (1:2000-1:5000 dilution). For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

At high antibody concentrations, a weak, non-specific band of ~30 kDa may appear in western blots.

Product Citations
  1. Perooli FM, et al. 2024. Sci Rep. 3066:14. PubMed
RRID
AB_2565215 (BioLegend Cat. No. 674402)

Antigen Details

Structure
483 amino acids with a predicted molecular weight of 54 kD and contains a DEAD box motif and a C-terminal helicase domain.
Distribution

Cytoplasm and P-bodies.

Function
DDX6 is a RNA-helicase with RNA-dependent ATP hydrolysis and ATP-dependent RNA unwinding activities that mediates mRNA translation, processing, and turnover.
Interaction
Interacts with LIMD1, WTIP, AJUBA, RC3H1, APOEBC3, AGO1, AGO2, and forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
Biology Area
Cell Biology
Antigen References

1. Pettersson OJ, et al. 2014. Nucleic Acids Res. 42:7186.
2. Huys A, et al. 2013. PLoS One 8:e67437.
3. Reed JC, et al. 2012. J. Cell Biol. 198:439.
4. Yu SF, et al. 2011. PLoS Pathog. 7:e1002303.
5. Bidet K, et al. 2011. RNA Biol. 6:1173.
6. Jangra RK, et al. 2010. J. Virol. 84:6810.

Gene ID
1656 View all products for this Gene ID
UniProt
View information about DDX6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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