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Purified anti-E. coli RNA Sigma S Antibody

Pricing & Availability
Clone
1RS1 (See other available formats)
Regulatory Status
RUO
Other Names
Sigma 38, rpoS, appR, katF, nur, otsX, sigS, RNA Polymerase
Isotype
Mouse IgG1
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Product Citations
publications
1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320
Whole cell extracts (15 µg protein) from E. coli DH5α cells grown to logarithmic (log) or stationary (stat) phase were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-E. coli RNA Sigma S antibody (clone 1RS1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S Red was used to confirm equal protein loading. Lane M: Molecular weight marker.
  • 1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320
    Whole cell extracts (15 µg protein) from E. coli DH5α cells grown to logarithmic (log) or stationary (stat) phase were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-E. coli RNA Sigma S antibody (clone 1RS1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S Red was used to confirm equal protein loading. Lane M: Molecular weight marker.
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663705 25 µg 81€
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663706 100 µg 203€
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Description

Sigma transcription factor controlling the regulation of genes is required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon, and other genes by modifying the affinity of RNA polymerase for the promoters.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# W0009 from Neoclone.

Clone 1RS1 does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthobacter, or Borrelia. It recognizes epitope 33-256.

Additional reported applications (for the relevant formats) include: functional assay.

Application References

(PubMed link indicates BioLegend citation)
  1. Nguyen LH, et al. 1993. Biochemistry 32:11112.
RRID
AB_2832841 (BioLegend Cat. No. 663705)
AB_2832841 (BioLegend Cat. No. 663706)

Antigen Details

Structure
Sigma S (σS) is a 38 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947210 View all products for this Gene ID
UniProt
View information about RNA Sigma S on UniProt.org
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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