- Clone
- W22099D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HAGH1, Hydroxyacylglutathione Hydrolase, Hydroxyacylglutathione Hydrolase, Mitochondrial, Glyoxalase II, GLO2, GLX2, GLO-2, Q16775
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
682951 | 25 µg | 144€ | ||||
682952 | 100 µg | 356€ |
Hydroxyacylglutathione hydrolase, mitochondrial or GLO2, is integral to cellular detoxification and metabolic regulation. It operates within the glyoxalase system, a critical pathway for neutralizing methylglyoxal, a highly reactive and cytotoxic byproduct of glycolysis. The glyoxalase system includes two primary enzymes: Lactoylglutathione lyase (GLO1) and HAGH/GLO2. GLO1 catalyzes the reaction between methylglyoxal and glutathione (GSH) to form S-D-lactoylglutathione. Subsequently, HAGH/GLO2 hydrolyzes S-D-lactoylglutathione to form D-lactate and regenerates GSH. This detoxification is crucial as methylglyoxal can cause significant cellular damage by glycation, which impairs the function of proteins, nucleic acids, and lipids. By mitigating this damage, HAGH helps protect cellular components and maintain cellular integrity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human HAGH (GLO2)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody (clone W22099D) is not suitable for immunocytochemistry (ICC).
For immunohistochemistry on formalin-fixed parafilm-embedded tissue (IHC-P), the recommended antigen retrieval is either Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704) or Citrate Buffer (Cat. No.420902). - RRID
-
AB_3662337 (BioLegend Cat. No. 682951)
AB_3662337 (BioLegend Cat. No. 682952)
Antigen Details
- Structure
- HAGH/GLO2 is a 308 amino acid protein with a predicted molecular weight of 34 kD.
- Distribution
-
Highly expressed in liver and kidney. Localizes in the cytosol and/or mitochondrial matrix.
- Function
- Catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid
- Biology Area
- Cell Biology, Mitochondrial Function
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Rabbani N, et al. 2014. Biochem Soc Trans. 42:419-24.
- Bangel FN, et al. 2015. Prog Neuropsychopharmacol Biol Psychiatry. 59:105-110.
- Gaffney DO, et al. 2020. Cell Chem Biol. 27:206-213.
- Gene ID
- 3029 View all products for this Gene ID
- UniProt
- View information about HAGH on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All HAGH Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HAGH | W22099D | WB,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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