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Purified anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
aJ099B12_WB_042612
Western blot analysis of cell lysates from HeLa cells transfected without or with His-tagged plasmid using His Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • aJ099B12_WB_042612
    Western blot analysis of cell lysates from HeLa cells transfected without or with His-tagged plasmid using His Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • bJ099B12_WB_042612
    Immunofluorescence of HeLa cells transfected without (A) or with (B) His-tagged protein plasmid using anti-His Mouse primary Antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B) is 1/100.
  • cJ099B12_IF_042612
    Insect cell supernatant expressing His-tagged mouse CCL22 was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-His Tag antibody (clone J099B12). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
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652501 25 µg 72€
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652502 100 µg 141€
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652505 1 mg 540€
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Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml (Dilution 1:1000 - 1:5000). For immunocytochemistry, a concentration range of 0.1 - 0.5 μg/ml (Dilution 1:1000 - 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  2. Goosens VJ, et al. 2021. ACS Synth Biol. 10:3422. PubMed
  3. Lam B, et al. 2021. Cell Rep. 37:109838. PubMed
  4. Shigemori S, et al. 2017. J Dairy Sci. 10.3168/jds.2017-12872. PubMed
  5. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  6. Namai F, et al. 2017. Curr Microbiol. 10.1007/s00284-017-1354-3. PubMed
  7. Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed
  8. Zoulias N, et al. 2021. Curr Biol. S0960-9822:01407. PubMed
  9. Williford JM, et al. 2019. Sci Adv. 5:eaay1357. PubMed
  10. Liu L, et al. 2019. J Cell Biol. 218:644. PubMed
  11. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  12. Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed
  13. Manrique-Suárez V, et al. 2021. Proteins. 89:1508. PubMed
  14. Yumoto K, et al. 2020. Antioxidants (Basel). 9:00. PubMed
  15. Keshwani M, et al. 2015. Biochem J. 472: 329 - 338. PubMed
RRID
AB_11204427 (BioLegend Cat. No. 652501)
AB_11204427 (BioLegend Cat. No. 652502)
AB_11204427 (BioLegend Cat. No. 652505)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org
Go To Top Version: 4    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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