Purified anti-human CD180 RP105 Antibody anti-CD180

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Clone: MHR73-11 (See other available formats)
Regulatory Status: RUO
Other Names: CD180, Radio-protective 105-kD protein, RP105
Isotype: Mouse IgG1, κ
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Product Citations: publications

MHR73-11_Purified_071607 — Human peripheral blood lymphocytes stained with purified MHR73-11, followed by anti-mouse IgG FITC

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312902

100 µg

156€

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Description

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human RP105 complexed with mouse MD-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
ICFC, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: intracellular staining¹, immunoprecipitation¹, immunohistochemistry¹ of acetone-fixed frozen tissue sections, B cell activation¹, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1)¹. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312913 and 312914).
RRID: AB_2228772 (BioLegend Cat. No. 312902)

Antigen Details

Structure: Member of the toll-like receptor family, 105 kD
Distribution: B cells, monocytes/macrophages, and dendritic cells
Function: Signaling protein involved in innate immunity
Ligand/Receptor: LPS
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Miura Y, et al. 1998. Blood 92:2815.
Gene ID: 4064 View all products for this Gene ID
UniProt: View information about CD180 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD180 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD180 (RP105)	MHR73-11	FC,ICFC,IHC-F,IP
PE anti-human CD180 (RP105)	MHR73-11	FC
PE/Cyanine7 anti-human CD180 (RP105)	MHR73-11	FC
APC anti-human CD180 (RP105)	MHR73-11	FC
Ultra-LEAF™ Purified anti-human CD180 (RP105)	MHR73-11	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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